Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/4307
Título: Rapid identification of differentially expressed genes by in situ screening of bacteria
Autor: Fonseca, V. G.
Lago-Lestón, A.
Laizé, Vincent
Cancela, Leonor
Palavras-chave: Differentially expressed genes
Suppression subtractive hybridization (SSH)
Data: Jun-2005
Editora: Humana Press
Citação: Fonseca VG; Lago-Lestón A; Laizé V; Cancela ML. Rapid identification of differentially expressed genes by in situ screening of bacteria, Molecular Biotechnology, 30, 2, 163-165, 2005.
Resumo: The identification of differentially expressed genes is a key step in the understanding of specific molecular mechanisms. Various methods have been developed to search for differences in expression but most of them are time or money consuming. We present here an alternative technique that connects standard suppression subtractive hybridization with in situ screening of bacteria to isolate and identify differentially expressed transcripts. The in situ differential screening is based on the transfer of bacteria directly from cultures onto nylon membranes with no need of phenol/chloroform extraction, colony lifting, or polymerase chain reaction amplification. This improved method was successfully applied and must be seen as a simple, low-cost, time-saving, and reproducible approach to identify differentially expressed genes.
Peer review: yes
URI: http://hdl.handle.net/10400.1/4307
DOI: http://dx.doi.org/ 10.1385/MB:30:2:163
ISSN: 1073-6085
Versão do Editor: http://link.springer.com/article/10.1385%2FMB%3A30%3A2%3A163
Aparece nas colecções:CCM2-Artigos (em revistas ou actas indexadas)



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