Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/6347
Título: PTEN Mediates the Antioxidant Effect of Resveratrol at Nutritionally Relevant Concentrations
Autor: Ingles, M.
Gambini, J.
Miguel, Maria Graça
Bonet Costa, V.
Abdelaziz, K. M.
El Alami, M.
Vina, J.
Borras, C.
Data: 2014
Editora: Hindawi Publishing Corporation
Resumo: Introduction. Antioxidant properties of resveratrol have been intensively studied for the last years, both in vivo and in vitro. Its bioavailability after an oral dose is very low and therefore it is very important to make sure that plasma concentrations of free resveratrol are sufficient enough to be active as antioxidant. Aims. In the present study, using nutritionally relevant concentrations of resveratrol, we aim to confirm its antioxidant capacity on reducing peroxide levels and look for the molecular pathway involved in this antioxidant effect. Methods. We used mammary gland tumor cells (MCF-7), which were pretreated with different concentrations of resveratrol for 48 h, and/or a PTEN inhibitor (bpV: bipy). Hydrogen peroxide levels were determined by fluorimetry, PTEN levels and Akt phosphorylation by Western Blotting, and mRNA expression of antioxidant genes by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results. Resveratrol treatment for 48 h lowered peroxide levels in MCF-7, even at low nutritional concentrations (1 nM). This effect was mediated by the activation of PTEN/Akt pathway, which resulted in an upregulation of catalase and MnSOD mRNA levels. Conclusion. Resveratrol acts as an antioxidant at nutritionally relevant concentrations by inducing the expression of antioxidant enzymes, through a mechanism involving PTEN/Akt signaling pathway.
Peer review: yes
URI: http://hdl.handle.net/10400.1/6347
DOI: https://dx.doi.org/10.1155/2014/580852
ISSN: 2314-6133
2314-6141
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

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