Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/6421
Título: In vitro spore germination of Polystichum drepanum, a threatened fern from Madeira Island
Autor: Chá-Chá, R.
Fernandes, F.
Romano, Anabela
Data: 2005
Editora: Headley Brothers
Resumo: Polystichum drepanum (Sw) C. Presl is a threatened fern endemic to a few forest areas in the north-west of Madeira Island. The aims of this work were to establish suitable culture conditions for in vitro germination of spores, and to evaluate short-term storage conditions for P drepanum spores. The highest frequency of germination was obtained in Murishage and Skoog (MS) liquid medium, without agitation. However, gametophytes maintained in MS liquid medium did not grow and, after 4 weeks, became anoxic and died. Thus, after germination in liquid medium, gametophytes were transferred to an MS double-phase culture system for further growth. The effects of storage period, temperature, and relative humidity during storage on in vitro spore germination were studied. Spore viability was assessed after 2, 4 and 6 months, and high viability (> 94%) was observed in all the assays. However, germination capability decreased with increased storage periods. The number of sporophytes obtained also decreased with prolonged storage periods. The results indicate that spores of R drepanum stored for 4 months at 21 degrees C maintain high viability and high germination frequency. ne sporophytes obtained were acclimatised in a mixture of peat and vermiculite [2:1 (v/v)] under high relative humidity (90-95%). Seventy-five sporophytes were successfully acclimatised to ex vitro conditions and showed active growth in the glasshouse.
Peer review: yes
URI: http://hdl.handle.net/10400.1/6421
ISSN: 1462-0316
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

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