Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/6492
Título: Physical mapping in a triplicated genome: mapping the downy mildew resistance locus Pp523 in Brassica oleracea L.
Autor: Carlier, J. D.
Alabaça, Cláudia S.
Sousa, N. H.
Coelho, P. S.
Monteiro, A. A.
Paterson, A. H.
Leitão, J. M.
Data: 2011
Editora: Genetics Society of America
Resumo: We describe the construction of a BAC contig and identification of a minimal tiling path that encompass the dominant and monogenically inherited downy mildew resistance locus Pp523 of Brassica oleracea L. The selection of BAC clones for construction of the physical map was carried out by screening gridded BAC libraries with DNA overgo probes derived from both genetically mapped DNA markers flanking the locus of interest and BAC-end sequences that align to Arabidopsis thaliana sequences within the previously identified syntenic region. The selected BAC clones consistently mapped to three different genomic regions of B. oleracea. Although 83 BAC clones were accurately mapped within a similar to 4.6 cM region surrounding the downy mildew resistance locus Pp523, a subset of 33 BAC clones mapped to another region on chromosome C8 that was similar to 60 cM away from the resistance gene, and a subset of 63 BAC clones mapped to chromosome C5. These results reflect the triplication of the Brassica genomes since their divergence from a common ancestor shared with A. thaliana, and they are consonant with recent analyses of the C genome of Brassica napus. The assembly of a minimal tiling path constituted by 13 (BoT01) BAC clones that span the Pp523 locus sets the stage for map-based cloning of this resistance gene.
Peer review: yes
URI: http://hdl.handle.net/10400.1/6492
DOI: https://dx.doi.org/10.1534/g3.111.001099
ISSN: 2160-1836
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

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