Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/6675
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Campo DCValorIdioma
dc.contributor.advisorRomano, Anabela-
dc.contributor.authorMartins, Neusa-
dc.date.accessioned2015-07-20T09:05:49Z-
dc.date.available2015-07-20T09:05:49Z-
dc.date.issued2012-
dc.date.submitted2012-
dc.identifier.urihttp://hdl.handle.net/10400.1/6675-
dc.description.abstractThis thesis aims to understand the aluminum (Al) bioaccumulation capacity, toxicity effects and tolerance mechanisms in two rare species endemic from Portugal, Plantago algarbiensis Samp. and Plantago almogravensis Franco. Firstly, two protocols were developed to propagate in vitro these species (Chapter 2). Afterwards it was investigated the influence of low pH on in vitro growth (Chapter 3). The seed germination requirements and the effects of Al on the germination and early development of seedlings were then evaluated in Chapters 4 and 5, respectively. The impact of low pH and Al on physiological responses, oxidative stress status and capacity for Al accumulation in micropropagated shoots and plantlets was investigated in the subsequent chapters (Chapters 6, 7 and 8). Finally, in Chapter 9 the possible implications of organic acids in Al detoxification were evaluated. Both species were successfully micropropagated showing high shoot multiplication rates and rooting frequencies and the micropropagated shoots of both species were able to tolerate and grow in low pH conditions. The seeds of both species germinate readily at 15 ºC and Al showed no impact on the germination percentage in either species but oxidative stress during early seedling development was detected. Although, both species accumulated considerable Al amounts and showed to be moderately tolerant to low pH and Al, P. almogravensis appeared to be more able to manage the oxidative stress and, therefore, adapted to maintain cellular physiology and growth under these stress conditions. Results suggested that Al detoxification in P. almogravensis implies both secretion of organic acids from roots and Al intracellular chelation by organic acids, while in P. algarbiensis only the internal mechanism seems to be involved. This research contributes to the understanding of the mechanisms underlying P. algarbiensis and P. almogravensis adaptation to acidic Al-rich conditions.por
dc.description.abstractO alumínio (Al) é o metal mais abundante na crosta terrestre, onde permanece maioritariamente insolúvel. Quando os solos se tornam ácidos, como é o caso de cerca de 40% do solo arável, o Al, dependendo do pH, pode hidrolisar-se em vários complexos que podem provocar diferentes efeitos biológicos. O complexo Al(H2O)6 3+ (ou simplesmente Al3+) é o mais fitotóxico, afetando o crescimento e o desenvolvimento das plantas. A sua toxicidade é explicada pela elevada afinidade do Al3+ para as paredes celulares, membranas plasmáticas e metabolitos, interferindo em importantes processos morfológicos, fisiológicos e moleculares. Apesar disso, algumas plantas desenvolveram mecanismos para combater o stress causado pelo Al sendo capazes de crescer em áreas com elevado teor deste metal. As espécies selvagens podem apresentar fenótipos mais tolerantes que as plantas cultivadas, e ser úteis para compreender os mecanismos de tolerância ao Al, fornecendo informação fundamental para aumentar a tolerância das espécies cultivadas.por
dc.description.sponsorshipUniversidade do Algarve, Faculdade de Ciências e Tecnologiapor
dc.language.isoengpor
dc.rightsopenAccesspor
dc.subjectSolospor
dc.subjectPropagação in vitropor
dc.subjectAlumíniopor
dc.subjectÁcidos orgânicospor
dc.subjectPlantago albarbiensispor
dc.subjectP. almogravensispor
dc.titleAluminum bioaccumulation and tolerance in Plantago algarbiensis Samp. and Plantago almogravensis Franco, assessed by using in vitro cultures and micropropagated plantspor
dc.typedoctoralThesispor
thesis.degree.disciplineCiências Biotecnológicas (Biotecnologia Vegetal)por
thesis.degree.levelDoutorpor
thesis.degree.nameDoutoramento em Ciências Biotecnológicas (Biotecnologia Vegetal)por
dc.identifier.tid101289774-
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