Browsing by Author "Oliveira, Ana R."
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- Assessment of European cuttlefish (Sepia officinalis, L.) nutritional value and freshness under ice storage using a developed Quality Index Method (QIM) and biochemical methodsPublication . Sykes, António V.; Oliveira, Ana R.; Domingues, Pedro M.; Cardoso, Carlos M.; Andrade, José Pedro; Nunes, Maria L.Cephalopod landings and consumption have been increasing worldwide during the past decades. The main reason for this increasing demand is that cephalopods are a good protein and lipid source (Kreuzer, 1984; Sinanoglou & Miniadis-Meimaroglou, 1998, 2000; Zlatanos, Laskaridis, Feist, & Sagredos, 2006), thus a highly nutritious food that represents an alternative to over exploited fish resources. Nutritional value of most Japanese squid and cuttlefish are described (Okuzumi & Fujii, 2000). Nevertheless, for the European cuttlefish (Sepia officinalis) as for most cephalopods of the Mediterranean and North Atlantic markets it is not yet well studied (Karakoltsidis, Zotos, & Constantinides, 1995; Ruı´z-Capillas, Moral, Morales, & Montero, 2002a).
- Microplastics presence in cultured and wild-caught cuttlefish, Sepia officinalisPublication . Oliveira, Ana R.; Sardinha-Silva, Andreia; Andrews, Paul L. R.; Green, Dannielle; Cooke, Gavan M.; Hall, Sarah; Blackburn, Kirsty; Sykes, Antonio V.Amongst cephalopods microplastics have been reported only in jumbo squid gut. We investigated microplastics in the digestive system of wild cuttlefish (Sepia officinalis) as they are predators and prey and compared the stomach, caecum/intestine and digestive gland (DG) of wild and cultured animals, exposed to seawater from a comparable source. Fibers were the most common type (approximate to 90% of total count) but were approximate to 2x higher in relation to body weight in wild vs. cultured animals. Fibers were transported to the DG where the count was approximate to 2x higher/g in wild (median 1.85 fibers/g) vs. cultured. In wild-caught animals the DG was the predominant location but in cultured animals the fibers were more evenly distributed in the digestive tract. the potential impact of mi-croplastics on health of cuttlefish is discussed. Cuttlefish represent a previously unrecognized source of micro plastic trophic transfer to fish and finding fibers in cultured animals has implications for aquaculture.
- Olfactory-like neurons are present in the forehead of common cuttlefish, Sepia officinalis Linnaeus, 1758 (Cephalopoda: Sepiidae)Publication . Campinho, Marco António; Oliveira, Ana R.; Sykes, António V.According to the literature, the cuttlefish, Sepia officinalis, possesses a specialized olfactory organ and cells, located in olfactory ventral pits. In this study, the location of olfactory receptor neurons (ORNs) at the cellular level was determined using cellular morphology and immunohistochemistry. An antiserum against PBP3 was used as a marker to identify ORN-like cells in cuttlefish after validation for specificity to cephalopod ORN cells in the common octopus, Octopus vulgaris. The results show that ORN-like cells in S. officinalis were not found in the ventral pits, suckers or the mouth lips. Instead, ORN-like cells were found scattered in the forehead, between the eyes. The absence of ORNlike cells in a pit in S. officinalis and the sharing of four similar types of ORN cells with the squid and octopus lineages suggest that this might be a later innovation in olfaction and is probably associated with the specialized lifestyle of these later evolved cephalopods. Together, this evidence suggests a diversification of ORN cell types in Coleoidea, which did not occur in Nautiloidea, which might have preceded the diversification of the Coleoidea.
- Reversion to developmental pathways underlies rapid arm regeneration in juvenile European cuttlefish, Sepia officinalis (Linnaeus 1758)Publication . Callaghan, Neal I.; Capaz, Juan Carlos; Lamarre, Simon G.; Bourloutski, Emilie; Oliveira, Ana R.; MacCormack, Tyson J.; Driedzic, William R.; Sykes, António V.Coleoid cephalopods, including the European cuttlefish (Sepia officinalis), possess the remarkable ability to fully regenerate an amputated arm with no apparent fibrosis or loss of function. In model organisms, regeneration usually occurs as the induction of proliferation in differentiated cells. In rare circumstances, regeneration can be the product of naive progenitor cells proliferating and differentiating de novo. In any instance, the immune system is an important factor in the induction of the regenerative response. Although the wound response is well-characterized, little is known about the physiological pathways utilized by cuttlefish to reconstruct a lost arm. In this study, the regenerating arms of juvenile cuttlefish, with or without exposure at the time of injury to sterile bacterial lipopolysaccharide extract to provoke an antipathogenic immune response, were assessed for the transcription of early tissue lineage developmental genes, as well as histological and protein turnover analyses of the resulting regenerative process. The transient upregulation of tissue-specific developmental genes and histological characterization indicated that coleoid arm regeneration is a stepwise process with staged specification of tissues formed de novo, with immune activation potentially affecting the timing but not the result of this process. Together, the data suggest that rather than inducing proliferation of mature cells, developmental pathways are reinstated, and that a pool of naive progenitors at the blastema site forms the basis for this regeneration.
- A sensory and nutritional comparison of mussels (Mytilus sp.) produced in NW Iberia and in the Armona offshore production area (Algarve, Portugal)Publication . Oliveira, Ana R.; Sykes, António V.; Hachero-Cruzado, Ismael; Azeiteiro, Ulisses M.; Esteves, E.A biometric, nutritional and sensory analysis of raw and cooked mussels comparing Mytilus sp. from the north-west coast of Portugal and Spain (Minho and Galicia, respectively) and the new offshore production site of Armona (Algarve, south Portugal) was carried out. In addition, multiple factorial analysis was performed to explore potential relationships between sensory attributes and nutritional content properties between the different mussels. Results showed that, at similar times of sale, biometrics of mussels from Armona and Vigo were similar and bigger than the remaining. Nonetheless, despite some similarities in proximate composition, mussels presented differences in lipid classes, fatty acid content and free amino acids profiles. These differences were not fully reflected in the sensory assessment by the panel, which were able to distinguish different production sites in raw specimens but displayed problems in discrimination these in cooked mussels. Some nutritional components were related to specific sensory sensations.