Browsing by Issue Date, starting with "2023-03-31"
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- O impacto da pandemia COVID-19 nos relacionamentos íntimos e satisfação com a vida em adultos portugueses: um estudo quantitativoPublication . Boryor, Catherine; Gomes, AlexandraEm dezembro de 2019, é identificado o vírus SARS-CoV-2, cuja doença foi posteriormente denominada COVID-19. Esta doença, com alto nível de transmissibilidade, longo período de incubação, e com pacientes assintomáticos obrigou à implementação de um período de quarentena, que forçou um confinamento social, com forte impacto no dia-a-dia da população. Períodos de confinamento diminuem o bem-estar e, consequentemente, a satisfação com a vida, aumentando o aparecimento de psicopatologias. As dinâmicas familiares alteraram inclusive a dinâmica conjugal, o que afeta a satisfação com a vida amorosa e a satisfação sexual. Assim, o presente estudo tem como objetivo avaliar dimensões do bem-estar psicológico, como a satisfação com a vida e os afetos positivos e negativos dos indivíduos durante o primeiro confinamento por COVID-19, bem como a satisfação com a vivência conjugal, nas dimensões afetivo-cognitiva e sexual, na população de adultos portugueses. Participaram 86 pessoas, destacando-se o emparelhamento de 19 casais, dos 18 aos 65 anos. Avaliámos os afetos, a satisfação com a vida, a satisfação com a vida amorosa, satisfação sexual e estratégias de coping diádico. Os principais resultados demonstram que durante o período de confinamento os casais, especialmente, os que proveem de agregados familiares maiores, experienciaram mais afetos negativo que, consequentemente, se reflete na sua satisfação com a vida. Durante o respetivo período, os afetos negativos, satisfação amorosa e sexual, e as estratégias de coping utilizadas pelos elementos do casal estão relacionadas positivamente. Já durante o período referente aos “últimos 15 dias”, esta relação é verificada entre satisfação com a vida amorosa, sexual e as estratégias de coping utilizadas, tendo os afetos negativos perdido expressão. Assim, é possível dizer que o confinamento pela pandemia de Covid-19 teve um impacto negativo, tanto na saúde mental dos adultos portugueses, como também, ao nível das suas dinâmicas conjugais.
- Next-Generation Sequencing (NGS) identified Species-Specific SSR and SNP markers, allow the unequivocal identification of Strawberry Tree (Arbutus unedo L.) germplasm accessions and contribute to assess their genetic relationshipsPublication . Pereira, Ricardo; Anjos, Isabela Vera Dos; M. Reis, João; Dias, Carolina; M Leitão, JThe strawberry tree (Arbutus unedo L.), an evergreen bush to small tree of the Ericaceae family, is a main component of the natural flora of the Mediterranean basin that also grows profusely through the Iberian Peninsula, southwestern France, and Ireland. The small edible red fruits are usually used to produce preserves, jams, and liquors, as the Portuguese “aguardente de medronho”. The leaves and fruits have been used for a long time in traditional medicine, and their bioactive compounds are presently the subject of intense research. A strawberry tree germplasm collection was recently established by the company Corte Velada (Odiáxere, Portugal). A set of 50 germplasm accessions was selected for a breeding program. A next-generation sequencing project was performed, resulting in the establishment of the first strawberry tree genome assembly and further identification of 500 SSR and 500 SNP loci. Individual molecular fingerprints for the unequivocal identification of the selected 50 accessions were established based on 71 markers alleles amplified by 4 SSR and 9 SNP markers. The same species-specific markers alleles combined with 61 random amplified markers amplified by 5 RAPD and 5 ISSR primers were used to assess the genetic variability and genetic relationships among the selected accessions.
- Unravelling the lipids content and the fatty acid profiles of eight recently described Halophytophthora Species and H. avicennae from the South Coast of PortugalPublication . Maia, Cristiana; Jung, Thomas; Engelen, Aschwin; Jung, Marília Horta; Custódio, LuísaIn this study, mycelia of eight recently described species of Halophytophthora and H. avicennae collected in Southern Portugal were analysed for lipids and fatty acids (FA) content to evaluate their possible use as alternative sources of FAs and understand how each species FAs profile relates to their phylogenetic position. All species had a low lipid percentage (0.06% in H. avicennae to 0.28% in H. frigida). Subclade 6b species contained more lipids. All species produced monounsaturated (MUFA), polyunsaturated (PUFA) and saturated (SFA) FAs, the latter being most abundant in all species. H. avicennae had the highest FA variety and was the only producer of γ-linolenic acid, while H. brevisporangia produced the lowest number of FAs. The best producer of arachidonic acid (ARA) and eicosapentaenoic acid (EPA) was H. thermoambigua with 3.89% and 9.09% of total FAs, respectively. In all species, palmitic acid (SFA) was most abundant and among the MUFAs produced oleic acid had the highest relative percentage. Principal component analysis (PCA) showed partial segregation of species by phylogenetic clade and subclade based on their FA profile. H. avicennae (Clade 4) differed from all other Clade 6 species due to the production of γ-linolenic and lauric acids. Our results disclosed interesting FA profiles in the tested species, adequate for energy (biodiesel), pharmaceutical and food industries (bioactive FAs). Despite the low amounts of lipids produced, this can be boosted by manipulating culture growth conditions. The observed interspecific variations in FA production provide preliminary insights into an evolutionary background of its production.