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Publication
Next-Generation Sequencing (NGS) identified Species-Specific SSR and SNP markers, allow the unequivocal identification of Strawberry Tree (Arbutus unedo L.) germplasm accessions and contribute to assess their genetic relationships
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Advisor(s)
Abstract(s)
The strawberry tree (Arbutus unedo L.), an evergreen bush to small tree of the Ericaceae
family, is a main component of the natural flora of the Mediterranean basin that also grows profusely
through the Iberian Peninsula, southwestern France, and Ireland. The small edible red fruits are
usually used to produce preserves, jams, and liquors, as the Portuguese “aguardente de medronho”.
The leaves and fruits have been used for a long time in traditional medicine, and their bioactive
compounds are presently the subject of intense research. A strawberry tree germplasm collection
was recently established by the company Corte Velada (Odiáxere, Portugal). A set of 50 germplasm
accessions was selected for a breeding program. A next-generation sequencing project was performed,
resulting in the establishment of the first strawberry tree genome assembly and further identification
of 500 SSR and 500 SNP loci. Individual molecular fingerprints for the unequivocal identification of
the selected 50 accessions were established based on 71 markers alleles amplified by 4 SSR and 9 SNP
markers. The same species-specific markers alleles combined with 61 random amplified markers
amplified by 5 RAPD and 5 ISSR primers were used to assess the genetic variability and genetic
relationships among the selected accessions.
Description
Keywords
Strawberry tree Arbutus unedo L Plant germplasm collection Genome assembly SSR markers SNP markers SNP-CAPS markers RAPD markers ISSR markers Plant molecular fingerprints Plant genetic relationships
Citation
Plants 12 (7): 1517 (2023)
Publisher
MDPI