Browsing by Issue Date, starting with "2024-11-28"
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- Questions éthiques dans la communication scientifiquePublication . Baptista Gil, FranciscoIn fact, we are currently witnessing a big change of organizations’ business models and the economic systems due to the great revolution in the realms of digital technology and artificial intelligence. This transformative period is defined by technological advancements and the creation of smart and intelligent systems, employing automation and fully digitalized methods (Muhuri et al., 2019). Digitalization, especially, AI, which can be defined as intelligence demonstrated by machines (computers and algorithms) in order to perform tasks and solve complex problems that would normally require or exceed the human intelligence, reasoning, and prediction power, is no longer affordable only by big firms, but also by very small firms and entrepreneurs. It offers to start-ups many new business opportunities and enhances their development. Digitalization in the finance field plays also a great contribution, it increasingly develops the finance services of organizations by establishing easily accessible digital financial platforms and broadening and deepening the scope of financial services (Giuggioli & Pellegrini, 2022). This international Symposium is being held, providing a forum for debate, scientific discussion and the sharing of experience on issues relating to Digital Transformation as it interacts with Finance, Innovation and Entrepreneurship.
- Improvement of Chlorella sorokiniana to increase the production of lipophilic compoundsPublication . Lourenço, Cristiana Isabel Rodrigues; Varela, João Carlos Serafim; Schüler, Lisa MaylinMicroalgae represent a promising alternative to terrestrial plants as a natural source of bioactive compounds with anti-inflammatory and antioxidant properties. These compounds can provide health benefits and help to prevent chronic diseases, such as inflammatory bowel disease (IBD), when incorporated into functional foods, nutraceuticals, and pharmaceuticals. However, large-scale microalgae production faces challenges in ensuring economic viability. Alongside optimising process parameters, strain improvement is a promising approach to enhance productivity and fine-tune the accumulation of target compounds. The Algae4IBD project aimed at developing novel algal-based products to treat IBD, identified lipophilic extracts from Chlorella sorokiniana with high anti-inflammatory activity. Therefore, the thesis aimed to increase the production of lipophilic compounds in C. sorokiniana under heterotrophic conditions. To accomplish this, chemically induced random mutagenesis using ethyl methanesulfonate (EMS) and screening with lipid pathway inhibitors led to the isolation of several inhibitor-resistant colonies. Among them, mutant M9 emerged as the most promising strain with a light green colour, which is considered more appealing for consumers, distinguishing itself from other mutants. This novel M9 strain exhibited a specific growth rate of 1.41 ± 0.01 d−1, biomass productivity of 2.02 ± 0.07 g L−1d−1 and biomass concentration of 6.85 ± 0.12 g L−1, similar to that of the WT. In addition, as quantified by BODIPY505/515 fluorescence in flow cytometry and gravimetry, its lipid content of 18.1 ± 0.3% DW was 50% higher than the WT's, 11.8 ± 1.20% DW. A preliminary two-stage cultivation involving the induction of lipophilic compounds, through exposure to 30 g L⁻¹ sea salt and/or 140 μmol m⁻² s⁻¹ of light, did not significantly increase lipid accumulation (p > 0.05), suggesting further optimization is needed. Despite this, the promising characteristics of mutant M9, particularly its higher lipid content, make it a strong candidate for further development in the commercial production of microalgae-based products.