Repository logo
 

Search Results

Now showing 1 - 10 of 52
  • Monitoring bubble production in a seagrass meadow using a source of opportunity
    Publication . Felisberto, Paulo; Rodríguez, Orlando; Silva, João P.; Jesus, Sergio; Ferreira, Hugo Q.; Ferreira, Pedro P.; Cunha, Maria E.; de los Santos, Carmen B.; Olivé, Irene; Santos, Rui
    Under high irradiance, the photosynthetic activity of dense seagrass meadows saturates the water forming oxygen bubbles. The diel cycle of bubble production peaks at mid-day, following light intensity pattern. It is well known that bubbles strongly affect the acoustic propagation, increasing signal attenuation and decreasing the effective water sound speed, noticeable at low frequencies. Thus, the diurnal variability of bubbles may show an interference pattern in the spectrograms of low frequency acoustic signals. In an experiment conducted in July 2016 at the Aquaculture Research Station of the Portuguese Institute for the Sea and Atmosphere in Olhão, Portugal, the spectrograms of low frequency (<20kHz) broadband noise produced by water pumps in a pond of 0.48ha covered by the seagrass Cymodocea nodosa showed interference patterns that can be ascribed to the variability of the sound speed in the water. Preliminary analysis suggests that the daily cycle of bubble concentration can be inferred from these interference patterns.
  • Effectiveness and consistency of a suite of descriptors to assess the ecological status of seagrass meadows (Posidonia oceanica L. Delile)
    Publication . Rotini, Alice; Belmonte, A.; Barrote, Isabel; Micheli, C.; Peirano, A.; Santos, Rui; Silva, João
    The increasing rate of human-induced environmental changes on coastal marine ecosystems has created a demand for effective descriptors, in particular for those suitable for monitoring the status of seagrass meadows. Growing evidence has supported the useful application of biochemical and genetic descriptors such as secondary metabolite synthesis, photosynthetic activity and genetic diversity. In the present study, we have investigated the effectiveness of different descriptors (traditional, biochemical and genetic) in monitoring seagrass meadow conservation status. The Posidonia oceanica meadow of Monterosso al Mare (Ligurian sea, NW Mediterranean) was subjected to the measurement of bed density, leaf biometry, total phenols, soluble protein and photosynthetic pigment content as well as to RAPD marker analysis. This suite of descriptors provided evidence of their effectiveness and convenient application as markers of the conservation status of P. oceanica and/or other seagrasses. Biochemical/genetic descriptors and those obtained by traditional methods depicted a well conserved meadow with seasonal variability and, particularly in summer, indicated a healthier condition in a portion of the bed (station C), which was in agreement with the physical and sedimentological features of the station. Our results support the usefulness of introducing biochemical and genetic approaches to seagrass monitoring programs since they are effective indicators of plant physiological stress and environmental disturbance.
  • Response of key stress-related genes of the seagrass Posidonia oceanica in the vicinity of submarine volcanic vents
    Publication . Lauritano, C.; Ruocco, M.; Dattolo, E.; Buia, M. C.; Silva, João; Santos, Rui; Olivé, Irene; Costa, M. M.; Procaccini, G.
    Submarine volcanic vents are being used as natural laboratories to assess the effects of increased ocean acidity and carbon dioxide (CO2) concentration on marine organisms and communities. However, in the vicinity of volcanic vents other factors in addition to CO2, which is the main gaseous component of the emissions, may directly or indirectly confound the biota responses to high CO2. Here we used for the first time the expression of antioxidant and stress-related genes of the seagrass Posidonia oceanica to assess the stress levels of the species. Our hypothesis is that unknown factors are causing metabolic stress that may confound the putative effects attributed to CO2 enrichment only. We analyzed the expression of 35 antioxidant and stress-related genes of P. oceanica in the vicinity of submerged volcanic vents located in the islands of Ischia and Panarea, Italy, and compared them with those from control sites away from the influence of vents. Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) was used to characterize gene expression patterns. Fifty-one percent of genes analyzed showed significant expression changes. Metal detoxification genes were mostly down-regulated in relation to controls at both Ischia and Panarea, indicating that P. oceanica does not increase the synthesis of heavy metal detoxification proteins in response to the environmental conditions present at the two vents. The up-regulation of genes involved in the free radical detoxification response (e.g., CAPX, SODCP and GR) indicates that, in contrast with Ischia, P. oceanica at the Panarea site faces stressors that result in the production of reactive oxygen species, triggering antioxidant responses. In addition, heat shock proteins were also activated at Panarea and not at Ischia. These proteins are activated to adjust stress-accumulated misfolded proteins and prevent their aggregation as a response to some stressors, not necessarily high temperature. This is the first study analyzing the expression of target genes in marine plants living near natural CO2 vents. Our results call for contention to the general claim of seagrasses as "winners" in a high-CO2 world, based on observations near volcanic vents. Careful consideration of factors that are at play in natural vents sites other than CO2 and acidification is required. This study also constitutes a first step for using stress-related genes as indicators of environmental pressures in a changing ocean.
  • Altered epiphyte community and sea urchin diet in Posidonia oceanica meadows in the vicinity of volcanic CO2 vents
    Publication . Nogueira, Patrícia; Gambi, Maria Cristina; Vizzini, Salvatrice; Califano, Gianmaria; Tavares, Ana Mafalda; Santos, Rui; Martinez-Cruz, Begona
    Ocean acidification (OA) predicted for 2100 is expected to shift seagrass epiphyte communities towards the dominance of more tolerant non-calcifying taxa. However, little is known about the indirect effects of such changes on food provision to key seagrass consumers. We found that epiphyte communities of the seagrass Posidonia oceanica in two naturally acidified sites (i.e. north and south sides of a volcanic CO2 vent) and in a control site away from the vent at the Ischia Island (NW Mediterranean Sea) significantly differed in composition and abundance. Such differences involved a higher abundance of non-calcareous crustose brown algae and a decline of calcifying polychaetes in both acidified sites. A lower epiphytic abundance of crustose coralline algae occurred only in the south side of the vents, thus suggesting that OA may alter epiphyte assemblages in different ways due to interaction with local factors such as differential fish herbivory or hydrodynamics. The OA effects on food items (seagrass, epiphytes, and algae) indirectly propagated into food provision to the sea urchin Paracentrotus lividus, as reflected by a reduced P. oceanica exploitation (i.e. less seagrass and calcareous epiphytes in the diet) in favour of non-calcareous green algae in both vent sites. In contrast, we detected no difference close and outside the vents neither in the composition of sea urchin diet nor in the total abundance of calcareous versus non-calcareous taxa. More research, under realistic scenarios of predicted pH reduction (i.e. <= 0.32 units of pH by 2100), is still necessary to better understand cascading effects of this altered urchin exploitation of food resources under acidified conditions on ecosystem diversity and function. (C) 2017 Elsevier Ltd. All rights reserved.
  • Using active and passive acoustics to assess O2 production of a Posidonia oceanica meadow
    Publication . Felisberto, P.; Zabel, F.; Rodríguez, O. C.; Santos, P.; Jesus, S. M.; Champenois, W.; Borges, A. V.; Santos, Rui
    This work discusses the data acquired during two experiments conducted in October 2011 and May 2013 in the Bay of la Revellata, Calvi, Corsica for the purpose of developing an acoustic system for monitoring the oxygen production of a seagrass meadow.
  • Correlation between the acoustic noise field measured in a Posidonia oceanica bed and the photosynthetic activity
    Publication . Felisberto, P.; Zabel, F.; Rodríguez, O. C.; Santos, P.; Jesus, S. M.; Champenois, W.; Borges, A. V.; Santos, Rui
    During the period of one week, from May 8 to 15, 2013, acoustic data was gathered at three locations over a Posidonia oceanica bed in the Bay of Revellata, Corsica. Preliminary analysis of the acoustic data shows that the environmental noise field in the band 2-7kHz was dominant during the period. The noise in this band is generally associated with wind and surface agitation. However, the noise power was not significantly correlated with wind speed. On the contrary, the diel cycle of the noise power at three locations was highly correlated with the water column concentration of O2, as measured by optodes. These measurements of environmental noise have confirmed the correlation between active acoustic signals transmitted along a seagrass meadow and the photosynthetic activity of the plants observed in a previous experiment conducted in the same area .The results suggest that acoustic noise can be used as a proxy for the photosynthetic oxygen production of a Posidonia oceanica meadow. Therefore, this work is a contribution for the development of a low cost passive acoustic system to assess the primary production of coastal ecosystems .
  • Ocean literacy to mainstream ecosystem services concept in formal and informal education: the example of coastal ecosystems of Southern Portugal
    Publication . Barracosa, Helena; de los Santos, Carmen B.; Martins, Márcio; Freitas, Cátia; Santos, Rui
    The concept of ecosystem services (ES) emerges as strategic to explain the influences that the ocean, and in particular coastal ecosystems, have on us and how we influence them back. Despite being a term coined several decades ago and being already widespread in the scientific community and among policy-makers, the ES concept still lacks recognition among citizens and educators. There is therefore a need to mainstream this concept in formal education and through Ocean Literacy resources. Although important developments in OL were done in the United States, particularly through the National Marine Educators Association (NMEA), this concept was only recently introduced in Europe. In Portugal, several informal OL education programs were developed in the last years, yet formal education on OL and, in particular, on ES is still very deficient. To address this limitation, the "Environmental Education Network for Ecosystem Services" (REASE), founded in 2017 in the Algarve region by a consortium of educational, environmental and scientific institutions, aims to increase OL through the dissemination of the perspective of how ES provided by coastal vegetation may contribute to the human well-being. The projects and activities implemented by REASE focus mostly on formal-education of school children and include: (1) capacity building for K-12 teachers, (2) educational programs to support and develop ES projects in schools, including a citizen science project to evaluate blue carbon stocks in the Algarve, (3) the publication of a children's book about the ES provided by the local Ria Formosa coastal lagoon, with a community-based participatory design (illustrations made by schoolchildren) and (4) a diverse array of informal education activities to raise awareness on the importance of coastal ecosystems on human well-being. REASE challenges are being successfully addressed by identifying threats to local coastal ecosystems that people worry about, and highlighting solutions to improve and maintain their health.
  • Light is more important than nutrient ratios of fertilization for cymodocea nodosa seedling development
    Publication . Alexandre, Ana; Silva, João; Santos, Rui
    Restoration of seagrass beds through seedlings is an alternative to the transplantation of adult plants that reduces the impact over donor areas and increases the genetic variability of restored meadows. To improve the use of Cymodocea nodosa seedlings, obtained from seeds germinated in vitro, in restoration programs, we investigated the ammonium and phosphate uptake rates of seedlings, and the synergistic effects of light levels (20 and 200 mu mol quanta m(-2) s(-1)) and different nitrogen to phosphorus molar ratios (40 mu M N:10 mu M P, 25 mu M N:25 mu M P, and 10 mu N:40 mu M P) on the photosynthetic activity and growth of seedlings. The nutrient content of seedlings was also compared to the seed nutrient reserves to assess the relative importance of external nutrient uptake for seedling development. Eighty two percent of the seeds germinated after 48 days at a mean rate of 1.5 seeds per day. All seedlings under all treatments survived and grew during the 4 weeks of the experiment. Seedlings of C. nodosa acquired ammonium and phosphate from the incubation media while still attached to the seed, at rates of about twice of adult plants. The relevance of external nutrient uptake was further highlighted by the observation that seedlings' tissues were richer in nitrogen and phosphorus than non-germinated seeds. The uptake of ammonium followed saturation kinetics with a half saturation constant of 32 mu M whereas the uptake of phosphate increased linearly with nutrient concentration within the range tested (5 - 100 mu M). Light was more important than the nutrient ratio of fertilization for the successful development of the young seedlings. The seedlings' photosynthetic and growth rates were about 20% higher in the high light treatment, whereas different nitrogen to phosphorus ratios did not significantly affect growth. The photosynthetic responses of the seedlings to changes in the light level and their capacity to use external nutrient sources showed that seedlings of C. nodosa have the ability to rapidly acclimate to the surrounding light and nutrient environment while still attached to the seeds. C. nodosa seedlings experiencing fertilization under low light levels showed slightly enhanced growth if nourished with a balanced formulation, whereas a slight increase in growth was also observed with unbalanced formulations under a higher light level. Our results highlight the importance of high light availability at the seedling restoration sites.
  • Nitrogen uptake in light versus darkness of the seagrass Zostera noltei: integration with carbon metabolism
    Publication . Alexandre, Ana; Silva, João; Santos, Rui
    We conducted a study that shows that light and dark conditions do not affect the uptake rates of ammonium and nitrate by the seagrass Zostera noltei. This is an important advantage over some seaweed species in which these rates are severely reduced at night. In the light, the ammonium uptake rates were initially higher (15 and 20molg(-1)h(-1)) and stabilized at a rate of 5molg(-1)h(-1) after 1h, whereas in the dark the rates remained constant at a rate of 10molg(-1)h(-1) over the first 180min of incubation. The rates of nitrate uptake in the light were high within the first 120min of incubation(7.2-11.1molg(-1)h(-1)) and decreased afterwards to lower values (0.8-3.9molg(-1)h(-1)), whereas in the dark the rates fluctuated around 0.0-11.1molg(-1)h(-1) throughout the whole incubation time (7h). The soluble sugar content of Z.noltei leaves increased significantly with both ammonium and nitrate incubations in the light, indicating the metabolic outcome of photosynthesis. In the dark, there was no significant variation in either the soluble sugar or in the starch content of leaves, rhizomes or roots in either the ammonium or nitrate incubations. However, the total starch content of plants decreased at night whereas the total soluble sugars increased, suggesting a process of starch catabolism to generate energy with the consequent production of smaller monosaccharide products. The starch content of rhizomes decreased significantly during the light incubations with nitrate but not with ammonium. These results suggest that carbohydrate mobilization is necessary for Z.noltei to account for extra energetic costs needed for the uptake and assimilation of nitrate. Furthermore, our results suggest that nitrate uptake, at least during the day, requires the mobilization of starch whereas the uptake of ammonium does not.
  • Physiological stress indicators in seagrasses
    Publication . Barrote, Isabel; Santos, Rui; Silva, João
    We investigated the combined effects of several environmental stressors in the biochemical defence mechanisms of the intertidal seagrass Zostera noltii and the subtidal seagrasses Zostera marina and Cymodocea nodosa in Ria Formosa coastal lagoon (southern Portugal). During one year Z. noltii was sampled for antioxidant enzymes activity, pigments, soluble protein and malondialdehyde (MDA) in both neap and spring tides. Intertidal seagrasses are periodically exposed to potentially stressful conditions such as desiccation, osmotic stress, high light and very high or freezing temperatures, depending on the season, latitude and tidal amplitude. In Ria Formosa Z. marina and C. nodosa appear frequently in the same meadow and compete for space, light and nutrients. These species are also exposed to stressful environmental conditions such as light or temperature stress and their survival depends on their relative ability to cope with all these stressors. In order to study the effects of low light in Z. marina and C. nodosa, four different levels of shading were imposed on field plots of both species during three weeks. P-I relationships were determined and samples were collected to determine photosynthetic pigments, non-structural carbohydrates, soluble protein, phenols and malondialdehyde concentration. Reactive oxygen species (ROS) are formed as a normal part of the plants metabolism. An increment on ROS formation is a common response to stress and can induce several types of damage, namely lipid peroxidation. MDA is a product of the peroxidation of membrane lipids and thus is commonly used as an indicator of oxidative stress, as is the concentration of phenolic compounds. Carotenoids and antioxidant enzymes are part of the plants’ antioxidative system. Higher content of carotenoids, besides amplifying the photosynthetic useful wavelengths, indicate higher photoprotection. Increased activities of ROS scavenging enzymes are correlated with stress tolerance. The relationships among experimental and environmental data will be discussed.