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  • Development of chondroid tissues in lordotic-kyphotic Senegalese sole (Solea senegalensis)
    Publication . Cardeira Da Silva, João; Mendes, A. C.; Pousão-Ferreira, P.; Cancela, Leonor; Gavaia, Paulo J.
    Vertebral deformities are frequent and constitute a major drawback for many aquaculture produced species. Lordosis and kyphosis, which are severe curvatures of the vertebral column, are particularly problematic, compromising fish marketing and profitability. Senegalese sole is a species with increasing interest in this industry and, like many others, presents high frequencies of vertebral deformities under rearing conditions.
  • Lordotic-kyphotic vertebrae develop ectopic cartilage-like tissue in Senegalese sole (Solea senegalensis)
    Publication . Cardeira Da Silva, João; Bensimon-Brito, A.; Pousão-Ferreira, P.; Cancela, Leonor; Gavaia, Paulo J.
    Lordosis and kyphosis are curvatures of the vertebral column that commonly affect Senegalese sole (Solea senegalensis) under intensive rearing conditions, constituting a problem for the aquaculture industry by increasing labor and operation costs and consequently reducing profits. However, no studies have yet addressed microanatomical changes in vertebral column curvature in this species. We have conducted histological analyses in order to determine the alterations at tissue and cellular levels in these deformities. Development of an ectopic cartilage-like tissue within bone matrix at the growth regions (contact area between two vertebrae) of affected vertebrae was observed in lordotic-kyphotic fish. As previously suggested, the increase in strain supported by deformed vertebrae may trigger the onset of metaplastic tissue formation through an osteochondroprogenitor precursor from the condensation of mesenchymal cells or by trans-differentiation of (pre-)osteoblastic cells to a chondrocytic lineage. The possibility of a notochord contribution, as well as the molecular events related to bone stress and bone types in terms of cellularity, are also discussed.
  • Early axial and appendicular skeletal development of the meagre (Argyrosomus regius). A comparison of two rearing systems
    Publication . Valles, E.; Cardeira Da Silva, João; Dionísio, Gisela; Estévez, A.; Pousão-Ferreira, P.; Cancela, Leonor; Gavaia, Paulo J.
    The meagre (Argyrosomus regius) is a new species for aquaculture with high economical value, presenting growth rates of 1 kg/year. Skeletal deformities generally appear in cultured larvae at early stages, reducing the value of the fish. The present study describes the osteological development of the vertebral column and fins in meagre larvae. Meagre larvae were obtained from wild broodstocks, kept at IRTA and IPIMAR, and induced to spawn using GnRHa injection.
  • Skeletal quality in Senegalese sole: characterization of fish resulting from environmental and nutritional trials
    Publication . Gavaia, Paulo J.; Dionísio, Gisela; Cardeira Da Silva, João; Cunha, M. E.; Pousão-Ferreira, P.; Conceição, L. E. C.; Cancela, Leonor
    The Senegalese sole (Solea senegalensis) is characteristic from southern Europe and Mediterranean. It has recently been adapted for aquaculture production since it is well accepted by consumers and reaches high commercial values. After the initial description of the ontogenic events of skeletogenesis, systematic evaluation of the incidence of skeletal malformations by several groups revealed high levels of skeletal deformities reaching up to 90% of the population.
  • Osteology of the axial and appendicular skeletons of the meagre Argyrosomus regius (Sciaenidae) and early skeletal development at two rearing facilities
    Publication . Cardeira Da Silva, João; Vallés, R.; Dionísio, Gisela; Estévez, A.; Gisbert, Enric; Pousão-Ferreira, P.; Cancela, Leonor; Gavaia, Paulo J.
    The meagre (Argyrosomus regius) is a new species for aquaculture diversification, presenting a high economic value and a high growth rate, reaching 1 kg year)1. The occurrence of deformities, which generally appear in cultured larvae at early stages, is problematic for aquaculture since they reduce fish fitness and profitability. Knowledge about the stages of skeletal formation is essential to accurately assess deformities in hatchery produced fish. Here, we describe the osteology of the axial skeleton and unpaired fins of the meagre. Based on this data, the osteological development of meagre larvae reared at the IPIMAR and IRTA-SCR, under different rearing conditions, was compared. Although little literature is available on the osteology of teleosts from the same taxonomic group, the skeletal composition of the meagre is comparable to that of other Sciaenidae. All skeletal structures considered appeared at earlier stages in larvae reared at IPIMAR, which presented a more advanced skeletal development in comparison to larvae of comparable length reared at IRTA-SCR that were submitted to lower temperature and light intensity, and reduced photoperiod regimes.
  • Early skeletal development of meagre (Argyrosomus regius, Asso 1801)
    Publication . Cardeira Da Silva, João; Nicolau, L.; Pousão-Ferreira, P.; Cancela, Leonor; Gavaia, Paulo J.
    The meagre (Argyrosomus regius) is a marine teleost with high growth rates and excellent biological characteristics for rearing, making it a proper candidate for diversification of aquaculture in Southern Europe. The description of skeletal development is essential to establish chronometric relationships between skeletal developments, types of ossification, processes and different development stages, contributing to the improvement of rearing conditions.