Characterization of recombinant desulfovibrio gigas ferredoxin

Rodrigues, Pedro; Graça, Fernando; Macedo, Anjos L.; Moura, Isabel; Moura, José J. G.

http://hdl.handle.net/10400.1/4361

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Abstract(s)

Dg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a [3Fe–4S] cluster per monomer. EPR and 1H NMR data of recombinant and wild-type protein are compared.

Keywords

Ferredoxin 3Fe–4S cluster Iron–sulfur cluster Paramagnetic protein Ferredoxin gene

URI

http://hdl.handle.net/10400.1/4361

Citation

Rodrigues, Pedro; Graça, Fernando; Macedo, Anjos L.; Moura, Isabel; Moura, José J.G. Characterization of Recombinant Desulfovibrio gigas Ferredoxin, Biochemical and Biophysical Research Communications, 289, 2, 630-633, 2001.