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Characterization of recombinant desulfovibrio gigas ferredoxin

2001Journal article Restricted access
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dc.contributor.authorRodrigues, Pedro
dc.contributor.authorGraça, Fernando
dc.contributor.authorMacedo, Anjos L.
dc.contributor.authorMoura, Isabel
dc.contributor.authorMoura, José J. G.
dc.date.accessioned2014-06-18T09:35:43Z
dc.date.available2014-06-18T09:35:43Z
dc.date.issued2001
dc.date.updated2014-06-12T13:47:46Z
dc.description.abstractDg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a [3Fe–4S] cluster per monomer. EPR and 1H NMR data of recombinant and wild-type protein are compared.por
dc.identifier.citationRodrigues, Pedro; Graça, Fernando; Macedo, Anjos L.; Moura, Isabel; Moura, José J.G. Characterization of Recombinant Desulfovibrio gigas Ferredoxin, Biochemical and Biophysical Research Communications, 289, 2, 630-633, 2001.por
dc.identifier.doihttp://dx.doi.org/10.1006/bbrc.2001.6016
dc.identifier.issn0006-291X
dc.identifier.otherAUT: PMR01268;
dc.identifier.urihttp://hdl.handle.net/10400.1/4361
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.idealibrary.compor
dc.subjectFerredoxinpor
dc.subject3Fe–4S clusterpor
dc.subjectIron–sulfur clusterpor
dc.subjectParamagnetic proteinpor
dc.subjectFerredoxin genepor
dc.titleCharacterization of recombinant desulfovibrio gigas ferredoxinpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage633por
oaire.citation.issue2por
oaire.citation.startPage630por
oaire.citation.titleBiochemical and Biophysical Research Communicationspor
oaire.citation.volume289por
person.familyNameRodrigues
person.givenNamePedro
person.identifier.orcid0000-0002-9668-1204
person.identifier.ridM-3406-2013
person.identifier.scopus-author-id55107531000
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationc4613fe6-e1a0-4dd9-be8d-442b2b8a7b72
relation.isAuthorOfPublication.latestForDiscoveryc4613fe6-e1a0-4dd9-be8d-442b2b8a7b72

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