Publicação
Assessment of cell disruption methods in an integrated multi-product biorefinery for Nannochloropsis Oceanica: from process design to economic analysis.
| datacite.subject.sdg | 07:Energias Renováveis e Acessíveis | |
| datacite.subject.sdg | 09:Indústria, Inovação e Infraestruturas | |
| datacite.subject.sdg | 12:Produção e Consumo Sustentáveis | |
| dc.contributor.author | Cunha, Pedro | |
| dc.contributor.author | Carvalho, Bernardo | |
| dc.contributor.author | Kholany, Mariam | |
| dc.contributor.author | Pereira, Hugo | |
| dc.contributor.author | Varela, João | |
| dc.date.accessioned | 2026-07-14T10:08:56Z | |
| dc.date.available | 2026-07-14T10:08:56Z | |
| dc.date.issued | 2026-07-14 | |
| dc.description.abstract | Microalgae are bioresources with significant potential within a sustainable, circular, bio-economy. However, high production costs have limited the widespread use of algae biomass. This study aimed to develop a multi-product biorefinery for Nannochloropsis oceanica that generates multiple revenue streams from the biomass, thereby enhancing the economic viability of algal production. The effectiveness of cell wall disruption using high-pressure homogenization and enzymatic hydrolysis was evaluated. Enzymatic hydrolysis solubilized nearly half (48.2 ± 1.5%) of the dry cell weight, compared to only 27.3 ± 3.2% with high-pressure homogenization, resulting in more concentrated water-soluble fractions and significantly higher protein extraction yields. Lipid extracts obtained after enzymatic hydrolysis had higher lipid (72.0 ± 5.3% w/w) and eicosapentaenoic acid (28.1 ± 6.9% w/w) contents than those from high-pressure homogenization (38.8 ± 6.1% w/w lipids; 9.1 ± 0.6% w/w eicosapentaenoic acid), despite similar lipid extraction yields (around 30%). Increasing the ethanol volumetric ratio from 58% to 75% v/v significantly improved lipid extraction yields (57.4 ± 3.1%) in the enzymatic hydrolysis-based biorefinery, with even higher yields observed upon scaling up (70.1%). All fractions, including lipid extracts, exhibited a balanced essential amino acid profile that exceeded the WHO/FAO/UNU-recommended values. A preliminary economic analysis indicated that lipid production was more cost-effective when cells were permeabilized by enzymatic hydrolysis than by high-pressure homogenization. | eng |
| dc.description.sponsorship | PhD fellowship (2021.08142.BD); UID/04326/2025; UID/PRR/04326/2025; UID/PRR/04326/2025); project “Pacto da Bioeconomia Azul” (Project No.C644915664—00000026) under WP5—Algae Vertical | |
| dc.identifier.doi | 10.3390/md24070245 | |
| dc.identifier.issn | 1660-3397 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/29263 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | MDPI | |
| dc.relation | Centre for Marine and Environmental Research | |
| dc.relation.ispartof | Marine Drugs | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Microalgae | |
| dc.subject | Biorefinery | |
| dc.subject | Multi-product | |
| dc.subject | Process integration | |
| dc.subject | Membrane filtration | |
| dc.subject | Ethanolic extraction | |
| dc.subject | Cost-effectiveness | |
| dc.title | Assessment of cell disruption methods in an integrated multi-product biorefinery for Nannochloropsis Oceanica: from process design to economic analysis. | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardNumber | LA/P/0101/2020 | |
| oaire.awardTitle | Centre for Marine and Environmental Research | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT | |
| oaire.citation.issue | 7 | |
| oaire.citation.startPage | 245 | |
| oaire.citation.title | Marine drugs | |
| oaire.citation.volume | 24 | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Carvalho | |
| person.familyName | Kholany | |
| person.familyName | Pereira | |
| person.familyName | Varela | |
| person.givenName | Bernardo | |
| person.givenName | Mariam | |
| person.givenName | Hugo | |
| person.givenName | João | |
| person.identifier.orcid | 0000-0003-4612-3797 | |
| person.identifier.orcid | 0000-0002-6279-2053 | |
| person.identifier.orcid | 0000-0002-1369-2099 | |
| person.identifier.orcid | 0000-0003-3101-693X | |
| person.identifier.rid | M-4223-2013 | |
| person.identifier.scopus-author-id | 55741416000 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
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