Cloning, characterization, and tissue distribution of prolactin receptor in the sea bream (Sparus aurata)

Santos, Cecilia; Ingleton, P. M.; Cavaco, J. E. B.; Kelly, P. A.; Edery, M.; Power, Deborah

http://hdl.handle.net/10400.1/5387

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Abstract(s)

The prolactin receptor (PRLR) was cloned and its tissue distribution characterized in adults of the protandrous hermaphrodite marine teleost, the sea bream (Sparus aurata). An homologous cDNA probe for sea bream PRLR (sbPRLR) was obtained by RT-PCR using gill mRNA. This probe was used to screen intestine and kidney cDNA libraries from which two overlapping clones (1100 and 2425 bp, respectively) were obtained.

Keywords

Prolactin receptor Marine teleost Osmoregulation Reproduction

URI

http://hdl.handle.net/10400.1/5387

Citation

C. R. A. Santos, P. M. Ingleton, J. E. B. Cavaco, P. A. Kelly, M. Edery and D. M. Power. Cloning, characterization, and tissue distribution of prolactin receptor in the sea bream (Sparus aurata). General and Comparative Endocrinology 121, 32–47 (2001).

Publisher

Elsevier

DOI

http://dx.doi.org/ 10.1006/gcen.2000.7553

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