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Immunochemical detection of parathyroid hormone-related protein in the saccus vasculosus of a teleost fish

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dc.contributor.authorDevlin, A. J.
dc.contributor.authorDanks, J. A.
dc.contributor.authorFaulkner, M. K.
dc.contributor.authorPower, Deborah
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorMartin, T. J.
dc.contributor.authorIngleton, P. M.
dc.date.accessioned2014-05-15T13:22:15Z
dc.date.available2014-05-15T13:22:15Z
dc.date.issued1996
dc.date.updated2014-05-13T10:33:42Z
dc.description.abstractUsing antisera to regions of human parathyroid hormone- related protein (PTHrP) the saccus vasculosus (SV) of the sea bream (Sparus aurata) has been shown to contain immunoreactive PTHrP. By immunohistochemistry (IHC) the epithelial coronet cells in fixed and waxembedded SV tissue reacted with antisera to the prepro region of human PTHrP (−13 to +2), the N-terminus PTHrP (1–16), and the midmolecule PTHrP (50–69). Sodium dodecyl sulfate–polyacrylamide gel electrophoresis of saccus extracts and incubation media contained two major proteins of 14.3 and 15 kDa. By Western blotting these two proteins both reacted with the three antisera used for IHC, suggesting that they are immunochemically similar to human PTHrP (1–84). Ultrastructurally the coronet cells of Sparus saccus vasculosus resembled coronet cells described for other teleosts, with an abundant smooth endoplasmic reticulum (SER) which was more highly organized in the coronets. IHC at EM level showed reaction mainly with the membranes of the SER. These results suggest that S. aurata saccus vasculosus may produce a PTHrP-like molecule similar to human PTHrP.por
dc.identifier.citationDevlin, A. J.; Danks, J. A.; Faulkner, M. K.; Power, D. M.; Canario, A. V. M.; Martin, T. J.; Ingleton, P. M.Immunochemical detection of parathyroid hormone-related protein in the saccus vasculosus of a teleost fish, General and Comparative Endocrinology, 101, 1, 83-90, 1996por
dc.identifier.issn0016-6480
dc.identifier.otherAUT: ACA00258; DPO00386
dc.identifier.urihttp://hdl.handle.net/10400.1/3959
dc.language.isoengpor
dc.peerreviewedyespor
dc.titleImmunochemical detection of parathyroid hormone-related protein in the saccus vasculosus of a teleost fishpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage90por
oaire.citation.startPage83por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume101por
person.familyNamePower
person.familyNameCanario
person.givenNameDeborah Mary
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521

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