Loading...
Publication
Immunochemical detection of parathyroid hormone-related protein in the saccus vasculosus of a teleost fish
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 1.4 MB | Adobe PDF |
Advisor(s)
Abstract(s)
Using antisera to regions of human parathyroid hormone-
related protein (PTHrP) the saccus vasculosus
(SV) of the sea bream (Sparus aurata) has been shown to
contain immunoreactive PTHrP. By immunohistochemistry
(IHC) the epithelial coronet cells in fixed and waxembedded
SV tissue reacted with antisera to the prepro
region of human PTHrP (−13 to +2), the N-terminus
PTHrP (1–16), and the midmolecule PTHrP (50–69). Sodium
dodecyl sulfate–polyacrylamide gel electrophoresis
of saccus extracts and incubation media contained
two major proteins of 14.3 and 15 kDa. By Western
blotting these two proteins both reacted with the three
antisera used for IHC, suggesting that they are immunochemically
similar to human PTHrP (1–84). Ultrastructurally
the coronet cells of Sparus saccus vasculosus resembled
coronet cells described for other teleosts, with
an abundant smooth endoplasmic reticulum (SER)
which was more highly organized in the coronets. IHC at
EM level showed reaction mainly with the membranes of
the SER. These results suggest that S. aurata saccus vasculosus
may produce a PTHrP-like molecule similar to
human PTHrP.
Description
Keywords
Citation
Devlin, A. J.; Danks, J. A.; Faulkner, M. K.; Power, D. M.; Canario, A. V. M.; Martin, T. J.; Ingleton, P. M.Immunochemical detection of parathyroid hormone-related protein in the saccus vasculosus of a teleost fish, General and Comparative Endocrinology, 101, 1, 83-90, 1996