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In vitro screening for estrogenic endocrine disrupting compounds using Mozambique tilapia and sea bass scales

2017-09Journal article Open access
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dc.contributor.authorPinto, Patricia
dc.contributor.authorEstêvão, M. Dulce
dc.contributor.authorSantos, Soraia
dc.contributor.authorAndrade, André
dc.contributor.authorPower, Deborah
dc.date.accessioned2019-11-20T15:07:24Z
dc.date.available2019-11-20T15:07:24Z
dc.date.issued2017-09
dc.description.abstractA wide range of estrogenic endocrine disruptors (EDCs) are accumulating in the environment and may disrupt the physiology of aquatic organisms. The effects of EDCs on fish have mainly been assessed using reproductive endpoints and in vivo animal experiments. We used a simple non-invasive assay to evaluate the impact of estrogens and EDCs on sea bass (Dicentrarchus labrax) and tilapia (Oreochromis mossambicus) scales. These were exposed to estradiol (E2), two phytoestrogens and six anthropogenic estrogenic/anti-estrogenic EDCs and activities of enzymes related to mineralized tissue turnover (TRAP, tartrate-resistant acid phosphatase and ALP, alkaline phosphatase) were measured. Semi-quantitative RT-PCR detected the expression of both membrane and nuclear estrogen receptors in the scales of both species, confirming scales as a target for E2 and EDCs through different mechanisms. Changes in TRAP or ALP activities after 30 minute and 24 h exposure were detected in sea bass and tilapia scales treated with E2 and three EDCs, although compound-, time- and dose-specific responses were observed for the two species. These results support again that the mineralized tissue turnover of fish is regulated by estrogens and reveals that the scales are a mineralized estrogen-responsive tissue that may be affected by some EDCs. The significance of these effects for whole animal physiology needs to be further explored. The in vitro fish scale bioassay is a promising non-invasive screening tool for E2 and EDCs effects, although the low sensitivity of TRAP/ALP quantification limits their utility and indicates that alternative endpoints are required.
dc.description.versioninfo:eu-repo/semantics/submittedVersion
dc.identifier.doi10.1016/j.cbpc.2017.06.002
dc.identifier.issn1532-0456
dc.identifier.urihttp://hdl.handle.net/10400.1/13022
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationE2SCALE: Scaling-down estrogenic responses in fish
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectTrout Oncorhynchus-Mykiss
dc.subjectLigand-binding domain
dc.subjectRainbow-trout
dc.subjectOsteoblastic activities
dc.subjectZebrafish scale
dc.subjectTeleost fish
dc.subjectBisphenol-A
dc.subjectOsteoclastic activities
dc.subjectBone metabolism
dc.subjectReceptor
dc.subjectAlkaline phosphatase
dc.subjectBioassay
dc.subjectEstradiol
dc.subjectFish scales
dc.subjectTartrate-resistant acid phosphatase
dc.titleIn vitro screening for estrogenic endocrine disrupting compounds using Mozambique tilapia and sea bass scales
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleE2SCALE: Scaling-down estrogenic responses in fish
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAG-GLO%2F4003%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F84033%2F2012/PT
oaire.citation.endPage113
oaire.citation.startPage106
oaire.citation.titleComparative Biochemistry and Physiology C-Toxicology & Pharmacology
oaire.citation.volume199
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNamePinto
person.familyNameEstêvão
person.familyNameSantos
person.familyNameAlçada Baptista Rebelo de Andrade
person.familyNamePower
person.givenNamePatricia IS
person.givenNameMaria Dulce da Mota Antunes de Oliveira
person.givenNameSoraia
person.givenNameAndré
person.givenNameDeborah Mary
person.identifier643457
person.identifierI-4695-2014
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.ciencia-id881B-53E8-0DB1
person.identifier.ciencia-idEF18-F0AF-D388
person.identifier.ciencia-idF513-56ED-7088
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0002-7151-8363
person.identifier.orcid0000-0001-5006-2021
person.identifier.orcid0000-0002-5900-8782
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-3817-2013
person.identifier.ridC-1328-2018
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id8381590400
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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