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Tissue responsiveness to estradiol and genistein in the sea bass liver and scale

2016-04Journal article Open access
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dc.contributor.authorEstêvão, M. Dulce
dc.contributor.authorAndrade, André
dc.contributor.authorSantos, Soraia
dc.contributor.authorPower, Deborah
dc.contributor.authorPinto, Patricia IS
dc.date.accessioned2017-04-07T15:56:54Z
dc.date.available2017-04-07T15:56:54Z
dc.date.issued2016-04
dc.description.abstractAs in mammals, estrogens in fish are essential for reproduction but also important regulators of mineral homeostasis. Fish scales are a non-conventional target tissue responsive to estradiol and constitute a good model to study mineralized tissues effects and mechanisms of action of estrogenic compounds, including phytoestrogens. The responsiveness to estradiol and the phytoestrogen genistein, was compared between the scales and the liver, a classical estrogenic target, in sea bass (Dicentrarchus labrax). Injection with estradiol and genistein significantly increased circulating vitellogenin (for both compounds) and mineral levels (estradiol only) and genistein also significantly increased scale enzymatic activities suggesting it increased mineral turnover. The repertoire, abundance and estrogenic regulation of nuclear estrogen receptors (ESR1, 2a and 2b) and membrane G-protein receptors (GPER and GPER-like) were different between liver and scales, which presumably explains the tissue-specific changes detected in estrogen-responsive gene expression. In scales changes in gene expression mainly consisted of small rapid increases, while in liver strong, sustained increases/decreases in gene expression occurred. Similar but not overlapping gene expression changes were observed in response to both estradiol and genistein. This study demonstrates for the first time the expression of membrane estrogen receptors in scales and that estrogens and phytoestrogens, to which fish may be exposed in the wild or in aquaculture, both affect liver and mineralized tissues in a tissue-specific manner. (C) 2015 Elsevier Ltd. All rights reserved.
dc.identifier.doihttp://dx.doi.org/10.1016/j.jsbmb.2015.12.023
dc.identifier.issn0960-0760
dc.identifier.otherAUT: MES00382; DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/9554
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationE2SCALE: Scaling-down estrogenic responses in fish
dc.relation.isbasedonWOS:000372690200014
dc.subjectEstrogenic compounds
dc.subjectEstrogen responsive genes
dc.subjectFish scales
dc.subjectG protein-coupled estrogen receptor
dc.subjectNuclear estrogen receptors
dc.subjectPhytoestrogens
dc.titleTissue responsiveness to estradiol and genistein in the sea bass liver and scale
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleE2SCALE: Scaling-down estrogenic responses in fish
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAG-GLO%2F4003%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F84033%2F2012/PT
oaire.citation.endPage137
oaire.citation.startPage127
oaire.citation.titleJournal of Steroid Biochemistry and Molecular Biology
oaire.citation.volume158
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNameEstêvão
person.familyNameAlçada Baptista Rebelo de Andrade
person.familyNameSantos
person.familyNamePower
person.familyNamePinto
person.givenNameMaria Dulce da Mota Antunes de Oliveira
person.givenNameAndré
person.givenNameSoraia
person.givenNameDeborah Mary
person.givenNamePatricia IS
person.identifierI-4695-2014
person.identifier643457
person.identifier.ciencia-id881B-53E8-0DB1
person.identifier.ciencia-idF513-56ED-7088
person.identifier.ciencia-idEF18-F0AF-D388
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.orcid0000-0002-7151-8363
person.identifier.orcid0000-0002-5900-8782
person.identifier.orcid0000-0001-5006-2021
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0001-7854-3898
person.identifier.ridC-1328-2018
person.identifier.ridM-3817-2013
person.identifier.scopus-author-id8381590400
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id10240774300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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