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Scrophularia lucida L. as a valuable source of bioactive compounds for pharmaceutical applications: In vitro antioxidant, anti-inflammatory, enzyme inhibitory properties, in silico studies, and HPLC profiles

2019-01Journal article Restricted
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dc.contributor.authorZengin, Gokhan
dc.contributor.authorStefanucci, Azzurra
dc.contributor.authorRodrigues, Maria Joao
dc.contributor.authorMollica, Adriano
dc.contributor.authorCustodio, Luisa
dc.contributor.authorAumeeruddy, Muhammad Zakariyyah
dc.contributor.authorMahomoodally, Mohamad Fawzi
dc.date.accessioned2020-07-24T10:53:02Z
dc.date.available2020-07-24T10:53:02Z
dc.date.issued2019-01
dc.description.abstractThe genus Scrophularia has received much interest with regards to its traditional uses against eczema, psoriasis, and mastitis. Yet, the medicinal properties of some species still need to be scientifically validated. The present study was designed to investigate into the biological properties of various solvent extracts (ethyl acetate, methanol, and aqueous) of the roots and aerial parts of Scrophularia lucida based on its antioxidant, anti-inflammatory, and enzyme inhibitory activities together with phytochemical screening. Our results revealed that the solvent extracts differed in their biological effectiveness. The root ethyl acetate extract showed the highest ABTS scavenging, FRAP, CUPRAC, and inhibitory activity against AChE and alpha-glucosidase. The ethyl acetate extract of the aerial parts displayed the highest BChE and alpha-amylase inhibition and antioxidant effect in the phosphomolybdenum assay, while the methanol extracts of both parts were the most effective DPPH center dot scavengers and tyrosinase inhibitors. The methanol extracts of the root and aerial parts also inhibited NO production in lipopolysaccharide (LPS)-stimulated murine leukemic monocyte-macrophage cell (4.99% and 10.77%, respectively), at 31.25 mu g/mL concentration. The highest TPC (34.98 mg GAE/g extract) and TFC (48.33 mg RE/g extract) were observed in the ethyl acetate extract of the root and aerial parts, respectively. The most abundant compounds in the root ethyl acetate extract were luteolin (852 mu g/g extract), rosmarinic acid (522 mu g/g extract), and hesperidin (394 mu g/g extract) while kaempferol was most abundant in the ethyl acetate extract of the aerial parts (628 mu g/g extract). In silico experiments were conducted on tyrosinase and the higher docking values were observed for rosmarinic acid and hesperidin. The present findings provide base line information which tend to support the potential use of S. lucida in the management of several chronic diseases, including Alzheimer's disease and diabetes mellitus. (C) 2018 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.jpba.2018.09.035
dc.identifier.issn0731-7085
dc.identifier.issn1873-264X
dc.identifier.urihttp://hdl.handle.net/10400.1/14439
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Science Bv
dc.subjectPhenylpropanoid glycosides
dc.subjectDifferent extracts
dc.subjectRosmarinic acid
dc.subjectProtein
dc.subjectTyrosinase
dc.subjectCanina
dc.subjectAssays
dc.subjectCholinesterase
dc.subjectProliferation
dc.subjectSpecificity
dc.titleScrophularia lucida L. as a valuable source of bioactive compounds for pharmaceutical applications: In vitro antioxidant, anti-inflammatory, enzyme inhibitory properties, in silico studies, and HPLC profiles
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage233
oaire.citation.startPage225
oaire.citation.titleJournal of Pharmaceutical and Biomedical Analysis
oaire.citation.volume162
person.familyNameRodrigues
person.givenNameMaria JoĂŁo
person.identifierR-004-VNG
person.identifier.ciencia-id2514-0E17-1D8D
person.identifier.orcid0000-0001-8732-710X
person.identifier.scopus-author-id56031608100
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication12c32925-de9e-4289-bdd5-1d655d7a278c
relation.isAuthorOfPublication.latestForDiscovery12c32925-de9e-4289-bdd5-1d655d7a278c

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