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DAX1 regulatory networks unveil conserved and potentially new functions

2013Journal article Restricted access
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dc.contributor.authorMartins, Rute S. T.
dc.contributor.authorPower, Deborah
dc.contributor.authorFuentes, J.
dc.contributor.authorDeloffre, Laurence A. M.
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-10-22T10:28:59Z
dc.date.available2014-10-22T10:28:59Z
dc.date.issued2013
dc.description.abstractDAX1 is an orphan nuclear receptorwith actions in mammalian sex determination, regulation of steroidogenesis, embryonic development and neural differentiation. Conserved patterns of DAX1 gene expression frommammals to fish have been taken to suggest conserved function. In the present study, the European sea bass, Dicentrarchus labrax, DAX1 promoter was isolated and its conserved features compared to other fish and mammalian DAX1 promoters in order to derive common regulators and functional gene networks. Fish andmammalian DAX1 promoters share common sets of transcription factor frameworkswhichwere also present in the promoter region of another 127 genes. Pathway analysis clustered these into candidate gene networks associated with the fish and mammalian DAX1. The networks identified are concordantwith described functions for DAX1 in embryogenesis, regulation of transcription, endocrine development and steroid production. Novel candidate gene network partners were also identified, which implicate DAX1 in ion homeostasis and transport, lipid transport and skeletal development. Experimental evidence is provided supporting roles for DAX1 in steroid signalling and osmoregulation in fish. These results highlight the usefulness of the in silico comparative approach to analyse gene regulation for hypothesis generation. Conserved promoter architecture can be used also to predict potentially newgene functions. The approach reported can be applied to genes from model and non-model species.por
dc.description.sponsorshipThe authors acknowledge Heiner Kuhl and Richard Reinhardt for sequencing the sea bass DAX1 and DAX2 promoters and to Pedro Guerreiro for his help in the salinity transfer experiment. The research leading to these results was funded by European Community's Seventh Framework Programme (FP7/2007–2013) under grant agreement no. 222719 - LIFECYCLE. RSTM and LAMD were in receipt, respectively, of fellowships SFRH/BPD/66742/2009 and PTDC/CVT/104750/2008 from the Portuguese Foundation for Science and Technology.por
dc.identifier.citationRute S.T. Martins, Deborah M. Power, Juan Fuentes, Laurence A.M. Deloffre, Adelino V.M. Canário. DAX1 regulatory networks unveil conserved and potentially new functions. Gene 530 (2013) 66–74.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.gene.2013.07.052
dc.identifier.otherAUT: DPO00386; ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/5400
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
dc.subjectDAX 1por
dc.subjectSteroidogenesispor
dc.subjectGene networkspor
dc.subjectPromoter architecturepor
dc.subjectTranscription binding sitespor
dc.subjectOsmoregulationpor
dc.titleDAX1 regulatory networks unveil conserved and potentially new functionspor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/222719/EU
oaire.citation.endPage74por
oaire.citation.startPage66por
oaire.citation.titleGenepor
oaire.citation.volume530por
oaire.fundingStreamFP7
person.familyNamePower
person.familyNameFuentes
person.familyNameCanario
person.givenNameDeborah Mary
person.givenNameJuan
person.givenNameAdelino
person.identifier392998
person.identifier143624
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id421B-E196-2C33
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0003-0430-8734
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id7201832526
person.identifier.scopus-author-id56568523700
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublicationd354b937-b18c-4ca5-a843-04a04269d60f
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relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0
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