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DAX1 regulatory networks unveil conserved and potentially new functions
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Orientador(es)
Resumo(s)
DAX1 is an orphan nuclear receptorwith actions in mammalian sex determination, regulation of steroidogenesis,
embryonic development and neural differentiation. Conserved patterns of DAX1 gene expression frommammals
to fish have been taken to suggest conserved function. In the present study, the European sea bass, Dicentrarchus
labrax, DAX1 promoter was isolated and its conserved features compared to other fish and mammalian DAX1
promoters in order to derive common regulators and functional gene networks. Fish andmammalian DAX1 promoters
share common sets of transcription factor frameworkswhichwere also present in the promoter region of
another 127 genes. Pathway analysis clustered these into candidate gene networks associated with the fish and
mammalian DAX1. The networks identified are concordantwith described functions for DAX1 in embryogenesis,
regulation of transcription, endocrine development and steroid production. Novel candidate gene network partners
were also identified, which implicate DAX1 in ion homeostasis and transport, lipid transport and skeletal
development. Experimental evidence is provided supporting roles for DAX1 in steroid signalling and osmoregulation
in fish. These results highlight the usefulness of the in silico comparative approach to analyse gene regulation
for hypothesis generation. Conserved promoter architecture can be used also to predict potentially newgene
functions. The approach reported can be applied to genes from model and non-model species.
Descrição
Palavras-chave
DAX 1 Steroidogenesis Gene networks Promoter architecture Transcription binding sites Osmoregulation
Contexto Educativo
Citação
Rute S.T. Martins, Deborah M. Power, Juan Fuentes, Laurence A.M. Deloffre, Adelino V.M. Canário. DAX1 regulatory networks unveil conserved and potentially new functions. Gene 530 (2013) 66–74.
Editora
Elsevier