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Development of a prototype of an aerosolization device for dry powders to improve in vitro cell-based assays in the context of lung delivery

2022-04Journal article Restricted access
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dc.contributor.authorPontes, Jorge Filipe
dc.contributor.authorHermínio P., Diogo
dc.contributor.authorConceição, Eusébio
dc.contributor.authorMusacchio, Flávia
dc.contributor.authorBorges dos Santos, R. M.
dc.contributor.authorGrenha, Ana
dc.date.accessioned2023-01-11T10:30:32Z
dc.date.available2023-01-11T10:30:32Z
dc.date.issued2022-04
dc.description.abstractThe lung has been, for the past years, subject of intense research for local and systemic drug delivery approaches. However, robust correlations between in vitro and in vivo results are often impaired, as in vitro experiments frequently have limitations mimicking lung conditions, especially when involving cell-based studies. This work proposes a 3D-printed device for aerosolization of dry powders over cells in culture, thus better resembling the conditions of aerosolization occurring in vivo. The proposed device comprises two parts, the weighing head, and the main body. Dry powders are weighed in the weighing head, which is then sealed to the main body. Next, compressed air is injected into the device, dragging the powder onto a plate, which fits the bottom of the main body. The plate is a suitable surface for cell culture. Dry powders (polysaccharide-based microparticles) were tested using the device, leading to an aerosolization yield up to 51%, which was observed to depend on the tested dry powder. The dry powder deposition profile is currently being evaluated using a Quartz-Crystal Microbalance, which replaces the plate at the bottom of the device’s main body. These experiments entail a more precise determination of the mass of aerosolized powders by analysing the differences in the vibration frequencies of a quartz crystal, thus allowing to assess the deposition profile. Although preliminary, the results show that the developed device may comprise an affordable solution for in vitro testing of dry powders when aerosolization over a surface is required.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.issn1941-2711
dc.identifier.urihttp://hdl.handle.net/10400.1/18787
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMary Ann Liebertpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relationStudy of the impact of dry powder insufflation over respiratory epithelial cells
dc.titleDevelopment of a prototype of an aerosolization device for dry powders to improve in vitro cell-based assays in the context of lung deliverypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleStudy of the impact of dry powder insufflation over respiratory epithelial cells
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/PD%2FBD%2F137064%2F2018/PT
oaire.citation.startPageA20-A20pt_PT
oaire.citation.titleJournal of aerosol medicine and pulmonary drug deliverypt_PT
oaire.citation.volume32pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
person.familyNamePontes
person.familyNameConceição
person.familyNameBorges dos Santos
person.familyNameGrenha
person.givenNameJorge Filipe
person.givenNameEusébio
person.givenNameRui M.
person.givenNameAna
person.identifier.ciencia-idCB16-5CBD-8B89
person.identifier.ciencia-id6317-FB21-9671
person.identifier.ciencia-id1819-C76F-A567
person.identifier.ciencia-id091C-0D58-7225
person.identifier.orcid0000-0002-6447-0242
person.identifier.orcid0000-0001-5963-2107
person.identifier.orcid0000-0002-4837-244X
person.identifier.orcid0000-0002-2136-1396
person.identifier.ridI-7931-2015
person.identifier.ridB-1816-2008
person.identifier.ridH-1392-2017
person.identifier.scopus-author-id57195033461
person.identifier.scopus-author-id6603299150
person.identifier.scopus-author-id6603430561
person.identifier.scopus-author-id8607930100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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