Detection of mineralized structures in very early stages of development of Marine Teleostei using a modified Alcian blue-Alizarin red double staining technique for bone and cartilage

Gavaia, Paulo J.; Sarasquete, C.; Cancela, Leonor

http://hdl.handle.net/10400.1/4193

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Authors

Gavaia, Paulo J.

Sarasquete, C.

Cancela, Leonor

Abstract(s)

We have developed a procedure for staining cartilage and bone in fish larvae as small as 2 mm (notochord length), for which standard alcian blue/alizarin red procedures did not give positive and/or consistent results. Small calcified structures only 100-200 pm in length can be clearly visualized. The method is suitable for both ontogenic studies during early stages of skeletal development in most marine fishes (e.g., Sparus aurata L., Solea senegalensis Kaup), whose larvae at hatching are often only a few millimeters long and for detecting skeletal abnormalities in small larvae. This procedure can also be used for specimens that have been preserved in 1000/0 ethanol for up to two years.

Keywords

Bone Alizarin red Alcian blue Diplodus sp. Cartilage Halobatrachus didactilus Larvae Skeleton Sotea senegalensis Sparus aurata

URI

http://hdl.handle.net/10400.1/4193

Citation

Gavaia, Paulo Jorge Travessa; Carmen Sarasquete; Leonor Cancela. Detection of mineralized structures in very early stages of development of Marine Teleostei using a modified Alcian blue-Alizarin red double staining technique for bone and cartilage, Biotechnic & Histochemistry , 75 , 79-84, 2000.