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Detection of mineralized structures in very early stages of development of Marine Teleostei using a modified Alcian blue-Alizarin red double staining technique for bone and cartilage

2000Journal article Restricted access
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dc.contributor.authorGavaia, Paulo J.
dc.contributor.authorSarasquete, C.
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-06-05T12:43:10Z
dc.date.available2014-06-05T12:43:10Z
dc.date.issued2000
dc.date.updated2014-05-30T10:47:01Z
dc.description.abstractWe have developed a procedure for staining cartilage and bone in fish larvae as small as 2 mm (notochord length), for which standard alcian blue/alizarin red procedures did not give positive and/or consistent results. Small calcified structures only 100-200 pm in length can be clearly visualized. The method is suitable for both ontogenic studies during early stages of skeletal development in most marine fishes (e.g., Sparus aurata L., Solea senegalensis Kaup), whose larvae at hatching are often only a few millimeters long and for detecting skeletal abnormalities in small larvae. This procedure can also be used for specimens that have been preserved in 1000/0 ethanol for up to two years.por
dc.identifier.citationGavaia, Paulo Jorge Travessa; Carmen Sarasquete; Leonor Cancela. Detection of mineralized structures in very early stages of development of Marine Teleostei using a modified Alcian blue-Alizarin red double staining technique for bone and cartilage, Biotechnic & Histochemistry , 75 , 79-84, 2000.por
dc.identifier.doihttp://dx.doi.org/10.3109/10520290009064151
dc.identifier.issn1052-0295
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/4193
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherInforma Healthcarepor
dc.subjectBonepor
dc.subjectAlizarin redpor
dc.subjectAlcian bluepor
dc.subjectDiplodus sp.por
dc.subjectCartilagepor
dc.subjectHalobatrachus didactiluspor
dc.subjectLarvaepor
dc.subjectSkeletonpor
dc.subjectSotea senegalensispor
dc.subjectSparus auratapor
dc.titleDetection of mineralized structures in very early stages of development of Marine Teleostei using a modified Alcian blue-Alizarin red double staining technique for bone and cartilagepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage84por
oaire.citation.issue2por
oaire.citation.startPage79por
oaire.citation.titleBiotechnic & Histochemistrypor
oaire.citation.volume75por
person.familyNameGavaia
person.familyNameCancela
person.givenNamePaulo
person.givenNameM. Leonor
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridA-6470-2011
person.identifier.scopus-author-id6507104377
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication9dca2139-21a4-4d59-aaf7-531f1033a58e
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication.latestForDiscovery9dca2139-21a4-4d59-aaf7-531f1033a58e

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