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Publicação
Identification of proteins with potential osteogenic activity present in the water-soluble matrix proteins from crassostrea gigas nacre using a proteomic approach
Orientador(es)
Resumo(s)
Nacre, when implanted in vivo in bones of dogs, sheep, mice, and humans, induces a biological response that includes integration and osteogenic activity on the host tissue that seems to be activated by a set of proteins present in the nacre water-soluble matrix (WSM). We describe here an experimental approach that can accurately identify the proteins present in the WSM of shell mollusk nacre. Four proteins (three gigasin-2 isoforms and a cystatin A2) were for the first time identified in WSM of Crassostrea gigas nacre using 2DE and LC-MS/MS for protein identification. These proteins are thought to be involved in bone remodeling processes and could be responsible for the biocompatibility shown between bone and nacre grafts. These results represent a contribution to the study of shell biomineralization process and opens new perspectives for the development of new nacre biomaterials for orthopedic applications.
Descrição
Palavras-chave
Oyster pinctada-fucata Tenascin-C Organic matrix Cathepsin-K Cysteine proteinases Bone-resorption Cystatin-C In-Vivo Pacific oyster Stefin-A
Contexto Educativo
Citação
Editora
Hindawi Publishing Corporation