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Identification of proteins with potential osteogenic activity present in the water-soluble matrix proteins from crassostrea gigas nacre using a proteomic approach

2012Journal article Open Access
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dc.contributor.authorOliveira, Daniel
dc.contributor.authorSilva, Tome S.
dc.contributor.authorCordeiro, Odete D.
dc.contributor.authorCavaco, Sofia I.
dc.contributor.authorSimes, Dina
dc.date.accessioned2018-12-07T14:58:31Z
dc.date.available2018-12-07T14:58:31Z
dc.date.issued2012
dc.description.abstractNacre, when implanted in vivo in bones of dogs, sheep, mice, and humans, induces a biological response that includes integration and osteogenic activity on the host tissue that seems to be activated by a set of proteins present in the nacre water-soluble matrix (WSM). We describe here an experimental approach that can accurately identify the proteins present in the WSM of shell mollusk nacre. Four proteins (three gigasin-2 isoforms and a cystatin A2) were for the first time identified in WSM of Crassostrea gigas nacre using 2DE and LC-MS/MS for protein identification. These proteins are thought to be involved in bone remodeling processes and could be responsible for the biocompatibility shown between bone and nacre grafts. These results represent a contribution to the study of shell biomineralization process and opens new perspectives for the development of new nacre biomaterials for orthopedic applications.
dc.description.sponsorshipPortuguese Science and Technology Foundation (FCT) [SFRH/BD/41392/2007]
dc.identifier.doi10.1100/2012/765909
dc.identifier.issn1537-744X
dc.identifier.urihttp://hdl.handle.net/10400.1/12065
dc.language.isoeng
dc.peerreviewedyes
dc.publisherHindawi Publishing Corporation
dc.subjectOyster pinctada-fucata
dc.subjectTenascin-C
dc.subjectOrganic matrix
dc.subjectCathepsin-K
dc.subjectCysteine proteinases
dc.subjectBone-resorption
dc.subjectCystatin-C
dc.subjectIn-Vivo
dc.subjectPacific oyster
dc.subjectStefin-A
dc.titleIdentification of proteins with potential osteogenic activity present in the water-soluble matrix proteins from crassostrea gigas nacre using a proteomic approach
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage765909
oaire.citation.titleScientific World Journal
person.familyNameOliveira
person.familyNameSimes
person.givenNameDaniel
person.givenNameDina
person.identifier222659
person.identifier.ciencia-idD414-8513-CFCA
person.identifier.orcid0000-0003-0622-2934
person.identifier.orcid0000-0002-5145-4753
person.identifier.ridN-2789-2014
person.identifier.scopus-author-id55253361100
person.identifier.scopus-author-id7201884723
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication18560e88-5df5-4bbc-8149-96b37dce1b47
relation.isAuthorOfPublication31bac96d-1d01-4b8f-8f2b-9da4be31ea41
relation.isAuthorOfPublication.latestForDiscovery31bac96d-1d01-4b8f-8f2b-9da4be31ea41

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