Evolution of the extracellular matrix in deuterostomes and the influence of calcitropic hormones

Martins, Florbela Alexandra Silva Vieira

Publication

Evolution of the extracellular matrix in deuterostomes and the influence of calcitropic hormones

2010Doctoral thesis

dc.contributor.advisor	Power, Deborah
dc.contributor.author	Martins, Florbela Alexandra Silva Vieira
dc.date.accessioned	2014-04-11T10:41:28Z
dc.date.available	2014-04-11T10:41:28Z
dc.date.issued	2010
dc.description	Tese de dout., Biologia (Biologia Molecular), Faculdade de Ciências e Tecnologia, Univ. do Algarve, 2010	por
dc.description.abstract	The extracellular matrix (ECM) is an essential component of all multicellular organisms. The present thesis characterized calcified tissue and its homeostasis considering ECM proteins and endocrine factors. From an evolutionary perspective homologues of mammalian calcified ECM genes were identified in teleost fish and aquatic bivalves (Crassostrea gigas and Laternula elliptica). Transcripts for collagens, fibronectin and thrombospondins and some factors with a possible role in calcium homeostasis and shell formation, e.g. calmodulin, bone morphogenetic protein (BMP) and calcitonin receptor-like (CLR) were identified in the bivalves. In the teleosts, sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax) ECM constituents, like collagens and adhesive proteins were identified as well as homologues of genes involved in human skeletal metabolism and 3 CLR were identified. The role of CLR in fish is ambiguous and in silico promoter analysis was used to infer possible function and suggested teleost CLR1 is most linked to the skeleton and CLR2 to reproduction. Experimental models to explore calcified tissue homeostasis included vertebra, jaws, and branchial arches. Treatment of sea bream with the Selective Estrogen-Receptor Modulator (SERM), raloxifene caused down-regulation of skeletal related transcripts (Alkaline Phosphatase (ALP), Core binding factor alpha 1(Cbfa1) and Cartilage Intermediate Layer protein (CILP)) in the branchial arches but not in vertebra and jaws. Regeneration of the integument (skin and scales) was studied with a sea bream oligo array. The wound rapidly repaired and within 3 days re-epithelization had occurred and the scale pocket was sealed and 7 days after scale removal a thin regenerated scale was visible. Major alterations of the transcriptome occurred at day 3 after scale removal and it had almost returned to basal levels by day 7. Global transcriptome changes during regeneration grouped under biological processes like cell cycle, tissue morphogenesis and immune physiology. Overall, the results provide knowledge to guide future functional studies of calcified tissue homeostasis in invertebrates and vertebrates.	por
dc.identifier.tid	101454945
dc.identifier.uri	http://hdl.handle.net/10400.1/3759
dc.language.iso	eng	por
dc.peerreviewed	yes	por
dc.subject	Biologia molecular	por
dc.subject	Análise comparativa	por
dc.subject	Evolução
dc.subject	Matrix extracelular
dc.subject	Regeneração
dc.title	Evolution of the extracellular matrix in deuterostomes and the influence of calcitropic hormones	por
dc.type	doctoral thesis
dspace.entity.type	Publication
rcaap.rights	restrictedAccess	por
rcaap.type	doctoralThesis	por