Publication
Green solutions: exploring nonsteroidal anti-inflammatory drugsĀ“ impact on cucurbitaceae for environmental remediation
| datacite.subject.fos | CiĆŖncias Naturais::Outras CiĆŖncias Naturais | |
| dc.contributor.advisor | Urbaniak, Magdalena | |
| dc.contributor.advisor | ChĆcharo, Luis | |
| dc.contributor.author | Mudiyanselage, Chathurika Priyadarshani Huladduwa | |
| dc.date.accessioned | 2025-05-14T15:13:55Z | |
| dc.date.available | 2025-05-14T15:13:55Z | |
| dc.date.issued | 2024-09-10 | |
| dc.description.abstract | Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used pharmaceuticals in the world, found as contaminants in water, soil, and sediment, posing risks to natural organisms and human health. Phytoremediation is an effective and ecologically acceptable approach for environmental pollutants. Plants from the Cucurbitaceae family have the potential to remediate contaminants in soil. Recent research indicates that certain fungicides can regulate the uptake of organic compounds, modulating major latex-like protein (MLP) in these plants. This study examines the impact of NSAIDs combined with benomyl on the physiology, biochemistry, and leaf endophytes of the zucchini plant (Cucurbita pepo). Plants were grown in OECD soil media under controlled greenhouse conditions for 28 days. There were six variants: control, paracetamol (25 mg/L), paracetamol + benomyl, diclofenac (2.5 mg/L), diclofenac + benomyl and benomyl. Water, fertilizer and benomyl were added according to a predefined schedule. After the incubation, fresh biomass, chlorophyll contents and phenolics concentrations were measured for each variant. Functional and structural diversity of leaf endophytes were analyzed by Biolog EcoPlateā¢ method and 16S rRNA gene sequencing. The study demonstrated that paracetamol decreased the fresh biomass of roots, stems and leaves, while diclofenac treatment had a similar trend, with the lowest stems and leaves biomass. Paracetamol treatment increased chlorophyll content, whereas diclofenac had a minimal effect on chlorophyll pigments. Additionally, phenolic compounds increased significantly in plants treated with paracetamol but lowered in the diclofenac variant compared to the control. Both NSAIDs significantly decreased the leaf endophytic metabolic activity and microbial structural diversity. Benomyl, when applied alone, also displayed some impacts on plant physiology and leaf endophytic microbial community. However, benomyl considerably mitigated the detrimental consequences of NSAID-treated plants by enhancing biomass and chlorophyll content, improving resilience to oxidative stress, and promoting endophytic microbial diversity. These findings contribute to a deeper understanding of the fungicide-mediated regulation of NSAID-induced phytotoxicity in zucchini and highlight the potential for developing strategies to enhance phytoremediation in contaminated environments. | eng |
| dc.description.abstract | Os anti-inflamatĆ³rios nĆ£o esterĆ³ides (AINEs) sĆ£o fĆ”rmacos amplamente utilizados em todo o mundo, encontrados como contaminantes na Ć”gua, no solo e nos sedimentos, representando riscos para os organismos naturais e para a saĆŗde humana. A fitorremediaĆ§Ć£o Ć© uma abordagem eficaz e ecologicamente aceitĆ”vel para a remediaĆ§Ć£o de poluentes ambientais. As plantas da famĆlia Cucurbitaceae tĆŖm o potencial de remediar contaminantes no solo. Pesquisas recentes indicam que certos fungicidas podem regular a absorĆ§Ć£o de compostos orgĆ¢nicos, modulando proteĆnas semelhantes a lĆ”tex (MLP) nestas plantas. Este estudo examina o impacto dos AINEs combinados com benomil na fisiologia, bioquĆmica e endĆ³fitos foliares da planta de curgete (Cucurbita pepo). As plantas foram cultivadas em substrato de solo OECD sob condiƧƵes controladas de estufa durante 28 dias. Foram utilizadas seis variantes: controlo, paracetamol (25 mg/L), paracetamol + benomil, diclofenac (2,5 mg/L), diclofenac + benomil e benomil. A Ć”gua, o fertilizante e o benomil foram adicionados de acordo com um cronograma predefinido. ApĆ³s a incubaĆ§Ć£o, foram medidos para cada variante a biomassa fresca, o conteĆŗdo de clorofila e as concentraƧƵes de compostos fenĆ³licos. A diversidade funcional e estrutural dos endĆ³fitos foliares foi analisada atravĆ©s do mĆ©todo Biolog EcoPlateā¢ e sequenciaĆ§Ć£o do gene 16S rRNA. O estudo demonstrou que o paracetamol diminuiu a biomassa fresca das raĆzes, caules e folhas, enquanto o tratamento com diclofenac apresentou uma tendĆŖncia similar, com a menor biomassa em caules e folhas. O tratamento com paracetamol aumentou o conteĆŗdo de clorofila, enquanto o diclofenac teve um efeito mĆnimo nos pigmentos de clorofila. AlĆ©m disso, os compostos fenĆ³licos aumentaram significativamente nas plantas tratadas com paracetamol, mas diminuĆram na variante com diclofenac em comparaĆ§Ć£o com o controlo. Ambos os AINEs diminuĆram significativamente a atividade metabĆ³lica endofĆtica foliar e a diversidade estrutural microbiana. O benomil, quando aplicado isoladamente, tambĆ©m demonstrou alguns impactos na fisiologia da planta e na comunidade microbiana endofĆtica foliar. No entanto, o benomil mitigou consideravelmente as consequĆŖncias prejudiciais das plantas tratadas com AINEs, ao aumentar a biomassa e o conteĆŗdo de clorofila, melhorando a resiliĆŖncia ao stress oxidativo e promovendo a diversidade microbiana endofĆtica. Estes resultados contribuem para uma compreensĆ£o mais profunda da regulaĆ§Ć£o mediada por fungicidas da fitotoxicidade induzida por AINEs na curgete e destacam o potencial para o desenvolvimento de estratĆ©gias para melhorar a fitorremediaĆ§Ć£o em ambientes contaminados. | por |
| dc.identifier.tid | 203875966 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/27117 | |
| dc.language.iso | eng | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | AINEs | |
| dc.subject | Benomil | |
| dc.subject | Fisiologia das plantas | |
| dc.subject | EndĆ³fitos foliares | |
| dc.subject | FitorremediaĆ§Ć£o | |
| dc.title | Green solutions: exploring nonsteroidal anti-inflammatory drugsĀ“ impact on cucurbitaceae for environmental remediation | eng |
| dc.type | master thesis | |
| dspace.entity.type | Publication | |
| thesis.degree.grantor | Universidade do Algarve. Faculdade de CiĆŖncias e Tecnologia | |
| thesis.degree.level | Mestre | |
| thesis.degree.name | Mestrado em Ecohidrologia Aplicada |