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Characterization of estrogen receptor ßb in sea bream (Sparus auratus): phylogeny, ligand-binding, and comparative analysis of expression

2006Journal article Restricted access
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dc.contributor.authorPinto, Patricia IS
dc.contributor.authorPassos, A. L.
dc.contributor.authorMartins, Rute S. T.
dc.contributor.authorPower, Deborah
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-05-13T10:40:51Z
dc.date.available2014-05-13T10:40:51Z
dc.date.issued2006
dc.date.updated2014-05-08T11:58:21Z
dc.description.abstractEstrogens control many physiological processes in both female and male vertebrates, mostly mediated by speciWc nuclear estrogen receptors (ER). Two ER subtypes (ER and ER ) are present in most vertebrates, including the sea bream (Sparus auratus) a hermaphrodite teleost Wsh. In the present study several variant cDNAs encoding a second sea bream ER (sbER b) is reported. Phylogenetic and Southern blot analysis indicate that sbER b and the previously cloned sbER a (formerly sbER ) are encoded by diVerent genes, which may have arisen by duplication of an ancestral ER gene. Competitive binding assays show that sbER b has high aYnity for 17 -estradiol (KdD1 nM) and speciWcally binds estrogen agonists (diethylstilbestrol and ethynylestradiol) and antagonists (ICI 182,780). In Northern blot sbER , sbER a, sbER b produce several diVerent transcripts in a variety of tissues. RT-PCR showed a partially overlapping but diVerential tissue distribution in both male and female sea bream.por
dc.identifier.citationPinto, Patrícia I.S.; Passos, Ana Lúcia; Martins, Deborah M. Power, Rute S.; Canário, Adelino V.M. Characterization of estrogen receptor ßb in sea bream (Sparus auratus): Phylogeny, ligand-binding, and comparative analysis of expression, General and Comparative Endocrinology, 145, 2, 197-207, 2006.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygcen.2005.08.010
dc.identifier.issn00166480
dc.identifier.otherAUT: DPO00386; ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3892
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectEstradiolpor
dc.subjectTeleost fishpor
dc.subjectAntagonistpor
dc.subjectAgonistpor
dc.subjectMultiple transcriptspor
dc.subjectReceptor bindingpor
dc.titleCharacterization of estrogen receptor ßb in sea bream (Sparus auratus): phylogeny, ligand-binding, and comparative analysis of expressionpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage207por
oaire.citation.issue2por
oaire.citation.startPage197por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume145por
person.familyNamePinto
person.familyNamePower
person.familyNameCanario
person.givenNamePatricia IS
person.givenNameDeborah Mary
person.givenNameAdelino
person.identifier643457
person.identifier143624
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridM-3817-2013
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication64bc526e-5281-44e5-91eb-5743436109f9
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery64bc526e-5281-44e5-91eb-5743436109f9

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