Characterization of estrogen receptor ßb in sea bream (Sparus auratus): phylogeny, ligand-binding, and comparative analysis of expression

Pinto, Patricia IS; Passos, A. L.; Martins, Rute S. T.; Power, Deborah; Canario, Adelino V. M.

http://hdl.handle.net/10400.1/3892

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Authors

Canario, Adelino V. M.

Abstract(s)

Estrogens control many physiological processes in both female and male vertebrates, mostly mediated by speciWc nuclear estrogen receptors (ER). Two ER subtypes (ER and ER ) are present in most vertebrates, including the sea bream (Sparus auratus) a hermaphrodite teleost Wsh. In the present study several variant cDNAs encoding a second sea bream ER (sbER b) is reported. Phylogenetic and Southern blot analysis indicate that sbER b and the previously cloned sbER a (formerly sbER ) are encoded by diVerent genes, which may have arisen by duplication of an ancestral ER gene. Competitive binding assays show that sbER b has high aYnity for 17 -estradiol (KdD1 nM) and speciWcally binds estrogen agonists (diethylstilbestrol and ethynylestradiol) and antagonists (ICI 182,780). In Northern blot sbER , sbER a, sbER b produce several diVerent transcripts in a variety of tissues. RT-PCR showed a partially overlapping but diVerential tissue distribution in both male and female sea bream.

Keywords

Estradiol Teleost fish Antagonist Agonist Multiple transcripts Receptor binding

URI

http://hdl.handle.net/10400.1/3892

Citation

Pinto, Patrícia I.S.; Passos, Ana Lúcia; Martins, Deborah M. Power, Rute S.; Canário, Adelino V.M. Characterization of estrogen receptor ßb in sea bream (Sparus auratus): Phylogeny, ligand-binding, and comparative analysis of expression, General and Comparative Endocrinology, 145, 2, 197-207, 2006.