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Liquid chromatography tandem mass spectrometry analysis of arachidonic and eicosapentanoic acid metabolites by using experimental design and curve resolution approaches
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Advisor(s)
Abstract(s)
Eicosanoids are important lipid mediators in the development of
inflammation, progression of cancer and other pain associated diseases.
Arachidonic acid (AA) is the precursor molecule for the synthesis of
prostaglandin E2 and luekotriene B4 which is considered as proinflammatory
metabolites. Conversely, eicosapentanoic acid (EPA) is the
substrate for the synthesis of anti-inflammatory metabolites such as
prostaglandin E3 and luekotriene B5.
In this study, the effects and influence of AA, EPA and inhibitor towards the
synthesis of the four metabolites in cell cultures were investigated. AAderived
metabolites showed minimal production when AA is the only
substrate present in the cell culture media. Interesting, the levels of AAderived
metabolites increased significantly in the presence of EPA. The
results showed that EPA may play a role in the production of the AAmetabolites.
An understanding of the effects and interactions between the
two molecules is important in establishing these metabolites as
inflammatory biomarkers.
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Keywords
Análises laboratoriais Cancro Moléculas orgânicas