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Phytochemical studies and biological activity of carnivorous plants from the Mediterranean region

2010Doctoral thesis Open access
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dc.contributor.advisorRomano, Anabela
dc.contributor.authorGrevenstuk, Tomás
dc.date.accessioned2012-09-27T13:50:32Z
dc.date.available2012-09-27T13:50:32Z
dc.date.issued2010
dc.descriptionTese de doutoramento, Ciências Biotecnológicas (Biotecnologia Vegetal), Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2010por
dc.description.abstractIn this thesis several studies were conducted with four carnivorous plant species which occur on Portuguese territory: Pinguicula lusitanica, Pinguicula vulgaris, Drosera intermedia and Drosera rotundifolia. Most habitats of these plants are threatened and natural populations are scarce, therefore micropropagation protocols were developed to produce biomass for the subsequent studies. Efficient micropropagation protocols were developed for P. lusitanica and D. intermedia enabling large scale biomass production, while protocols for the other two species have still to be optimized (in Chapter 2). The in vitro established cultures represent active germplasm collections of Portuguese natural populations and contribute therefore for their conservation. In Chapter 3 extracts prepared from micropropagated plant material were analyzed using state of the art HPLC-ESI-MS and HPLC-SPE-NMR equipment which enabled the identification of the major secondary metabolites produced by P. lusitanica and D. intermedia, directly from essentially crude extracts. The metabolites identified in P. lusitanica belong to the iridoid glucosides and caffeoyl phenylethanoid glycosides and D. intermedia was shown to produce mainly flavonoid glucosides, ellagic acid derivatives and the naphthoquinone plumbagin. The evaluation of the biological activities of these extracts, compiled in Chapter 4, showed that the methanol extract of P. lusitanica has considerable antioxidant activity and that the n-hexane extract of D. intermedia has high antimicrobial potential. In Chapter 5 a method for the extraction of plumbagin from micropropagated D. intermedia plants was optimized and its potential as an alternative for bioprospection evaluated. It was shown that the commercial exploitation of plumbagin from D. intermedia cultures might be viable and that UAE with n-hexane followed by an SPE purification step is an efficient procedure for obtaining large quantities of high purity plumbagin. It is hoped that this study represents an enrichment of the knowledge on these plants and contributes to their conservation and valorisation.por
dc.identifier.other57.08 GRE*Phy Cave
dc.identifier.tid101246439
dc.identifier.urihttp://hdl.handle.net/10400.1/1684
dc.language.isoengpor
dc.peerreviewedyespor
dc.relationPHYTOCHEMICAL STUDIES AND BIOLOGICAL ACTIVITY OF INSECTIVOROUS PLANTS FROM THE MEDITERRANEAN REGION
dc.subjectPinguiculapor
dc.subjectDroserapor
dc.subjectMicropropagationpor
dc.subjectConservationpor
dc.subjectHyphenated analytical techniquespor
dc.subjectAntioxidant activitypor
dc.subjectAntimicrobial activitypor
dc.subjectBioprospectionpor
dc.subjectPlumbaginpor
dc.titlePhytochemical studies and biological activity of carnivorous plants from the Mediterranean regionpor
dc.typedoctoral thesis
dspace.entity.typePublication
oaire.awardTitlePHYTOCHEMICAL STUDIES AND BIOLOGICAL ACTIVITY OF INSECTIVOROUS PLANTS FROM THE MEDITERRANEAN REGION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F31777%2F2006/PT
person.familyNameGrevenstuk
person.givenNameTomás
person.identifier.ciencia-idB31A-EC35-5A31
person.identifier.orcid0000-0003-0779-3018
person.identifier.scopus-author-id24329026400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typedoctoralThesispor
relation.isAuthorOfPublication4ae6491f-f21f-4001-8987-dec1ad73ca5c
relation.isAuthorOfPublication.latestForDiscovery4ae6491f-f21f-4001-8987-dec1ad73ca5c
relation.isProjectOfPublication7ebb3697-c5c1-4d12-8387-8c558c6a928e
relation.isProjectOfPublication.latestForDiscovery7ebb3697-c5c1-4d12-8387-8c558c6a928e
thesis.degree.grantorUniversidade do Algarve. Faculdade de Ciências e Tecnologiapor
thesis.degree.levelDoutorpor
thesis.degree.nameDoutoramento em Ciências Biotecnológicas. Biotecnologia Vegetalpor

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