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In vitro and in vivo quantification of elicitin expression in Phytophthora cinnamomi
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Orientador(es)
Resumo(s)
The differential expression of four Phytophthora cinnamomi elicitin genes was analysed by Real Time
RT-PCR. In in vitro cultures, the a-cinnamomin gene showed the highest level of expression, the
b-cinnamomin gene (b-cin) was the most inducible, and the HAE transcripts were in low abundance.
Transcription of all the elicitins was active during the active growth of the pathogen when infecting
cork oak (Quercus suber) roots, and as host colonization progressed, the level of b-cin expression fell,
while that of a-cin rose. In an antisense transgenic strain, the silencing of b-cin also negatively affected
the expression of other elicitin genes in the cluster. The reduced in planta growth of the b-cin knock-out
is related to the altered pattern of elicitin gene expression, supporting the idea that one of the functions
of elicitins is related, directly or indirectly, with pathogenesis.
Descrição
Palavras-chave
Phytophthora cinnamomi Elicitin expression Pathogenesis Transformation Gene silencing Quercus
Contexto Educativo
Citação
Horta, M.; Sousa, Nelson; Coelho, A. C.; Neves, D.; Cravador, Alfredo. In vitro and in vivo quantification of elicitin expression in Phytophthora cinnamomi. Physiological and Molecular Plant Pathology, 73, 1-3, 48-57, 2008.