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In vitro and in vivo quantification of elicitin expression in Phytophthora cinnamomi

2008Journal article Restricted access
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dc.contributor.authorHorta, Marília
dc.contributor.authorSousa, Nelson
dc.contributor.authorCoelho, A. C.
dc.contributor.authorNeves, D.
dc.contributor.authorCravador, A.
dc.date.accessioned2012-05-16T09:01:04Z
dc.date.available2012-05-16T09:01:04Z
dc.date.issued2008
dc.date.updated2012-05-12T23:21:01Z
dc.description.abstractThe differential expression of four Phytophthora cinnamomi elicitin genes was analysed by Real Time RT-PCR. In in vitro cultures, the a-cinnamomin gene showed the highest level of expression, the b-cinnamomin gene (b-cin) was the most inducible, and the HAE transcripts were in low abundance. Transcription of all the elicitins was active during the active growth of the pathogen when infecting cork oak (Quercus suber) roots, and as host colonization progressed, the level of b-cin expression fell, while that of a-cin rose. In an antisense transgenic strain, the silencing of b-cin also negatively affected the expression of other elicitin genes in the cluster. The reduced in planta growth of the b-cin knock-out is related to the altered pattern of elicitin gene expression, supporting the idea that one of the functions of elicitins is related, directly or indirectly, with pathogenesis.por
dc.identifier.citationHorta, M.; Sousa, Nelson; Coelho, A. C.; Neves, D.; Cravador, Alfredo. In vitro and in vivo quantification of elicitin expression in Phytophthora cinnamomi. Physiological and Molecular Plant Pathology, 73, 1-3, 48-57, 2008.por
dc.identifier.issn0885-5765
dc.identifier.otherAUT: ACR00659; ACO00215;
dc.identifier.urihttp://hdl.handle.net/10400.1/1173
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectPhytophthora cinnamomipor
dc.subjectElicitin expressionpor
dc.subjectPathogenesispor
dc.subjectTransformationpor
dc.subjectGene silencingpor
dc.subjectQuercuspor
dc.titleIn vitro and in vivo quantification of elicitin expression in Phytophthora cinnamomipor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3por
oaire.citation.issue73por
oaire.citation.startPage1por
oaire.citation.titlePhysiological and Molecular Plant Pathologypor
person.familyNameHorta Jung
person.familyNameCravador
person.givenNameMarília
person.givenNameAlfredo
person.identifier.orcid0000-0003-2219-8647
person.identifier.orcid0000-0002-9831-9815
person.identifier.ridE-7624-2013
person.identifier.ridK-7247-2012
person.identifier.scopus-author-id16312454300
person.identifier.scopus-author-id6602537257
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication2fee8729-b025-4c5e-bc83-397229a037ef
relation.isAuthorOfPublication8c84a9e6-6b7f-4b50-93a2-152c85901722
relation.isAuthorOfPublication.latestForDiscovery8c84a9e6-6b7f-4b50-93a2-152c85901722

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