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Antioxidant, cytotoxic and apoptotic activity of Drosophyllum lusitanicum extracts
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Advisor(s)
Abstract(s)
This study was designed to determine the antioxidant, anti-proliferative, cytotoxic and apoptogenic properties of aqueous, methanol and hexane extracts from Drosophyllum lusitanicum leaves. Assays showed that the methanol extract had the highest antioxidant activity (F-C: 1188.06 ± 52.96 μmolGAE/mgextract; TEAC: 432.23 ± 6.71 μmolTE/mgextract; ORAC: 764.18 ± 61.18 μmolTE/mgextract)and the hexane extract had the lowest (F-C: 20.16 ± 1.62 μmolGAE/mgextract; TEAC: 20.94 ± 1.73
μmolTE/mgextract; ORAC: 50.66 ± 0.91 μmolTE/mgextract). The effect of plant extracts on HeLa cells proliferation and viability was evaluated by the crystal violet and WST-1 assays, respectively and
apoptotic cells were determined using PI staining of DNA fragmentation by flow cytometry (sub-G1 peak). It was observed that the methanol extract inhibited cell proliferation and induced apoptosis on
human cervical adenocarcinoma HeLa cells. Moreover, results indicated that the D. lusitanicum hexane extract exhibited cytotoxic effects on HeLa cells via apoptosis and also arrested cell cycle progression in G2/M phase. As damaging events are frequently correlated with oxidative stress, the prevalence of both antioxidant and anti-proliferative properties in the same plant species could be beneficial in terms of rational, preventive or therapeutic purposes. Thus D. lusitanicum may be a potential candidate in the field of anticancer drug discovery.
Description
Keywords
Anti-proliferation Apoptosis Cell cycle HeLa cells Carnivorous plant Viability
Citation
Gonçalves S., Xavier C., Alberício F., Costa P., Romano A. 2010. Antioxidant, cytotoxic and apoptotic activity of Drosophyllum lusitanicum extracts, Journal of Medicinal Plants Research 4: 1601-1608