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Biological effects of decavanadate: muscle contraction, in vivo oxidative stress, and mitochondrial toxicity

dc.contributor.authorAureliano, M.
dc.date.accessioned2012-06-26T10:51:50Z
dc.date.available2012-06-26T10:51:50Z
dc.date.issued2007
dc.description.abstractDecameric vanadate species (V10) can be formed at physiological pH values in vanadate solutions presumably containing only monomeric vanadate species (V1). Sarcoplasmic reticulum Ca2+-ATPase and myosin are known to interact with decameric vanadate species. V10 interaction with myosin is favored by conformational changes that takes place in myosin during the catalytic cycle. Apparently, V10 operates at a different protein state in comparison to monomeric vanadate (V1) that mimics the protein at the hydrolysis transition state. V10 also clearly differs from V1, by inhibiting sarcoplasmic reticulum calcium accumulation in non-damage native vesicles, besides affecting calcium efflux associated with ATP synthesis and proton ejection associated with ATP hydrolysis. Recently reported studies referred that V10 is stabilized by actin during the process of the protein polymerization since the decomposition half-life time increases from 5 to 27 hours, suggesting that the interaction is also support by a protein conformation induce during ATP hydrolysis followed by the formation of protein filaments. Besides affecting muscle contraction and its regulation, V10, as low as 100 nM, inhibits 50% of oxygen consumption in mitochondria, pointing that this organelle is a potential cellular target for V10, while a 100-fold higher concentration of V1 (10 µM) is needed to induce the same effect. Furthermore, in vivo studies have shown that following an acute exposure, decavanadate induced different changes, when compared to vanadate, on oxidative stress markers, vanadium intracellular accumulation as well as in lipid peroxidation. Putting it all together, it is suggested that the biological effects of decameric vanadate species contribute, at least in part, to the understanding of the versatility of vanadium biochemistry.por
dc.identifier.isbn9780841274464
dc.identifier.urihttp://hdl.handle.net/10400.1/1306
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmerican Chemical Society Symposium seriespor
dc.subjectDecavanadatepor
dc.subjectVanadiummetallomicspor
dc.titleBiological effects of decavanadate: muscle contraction, in vivo oxidative stress, and mitochondrial toxicitypor
dc.typebook part
dspace.entity.typePublication
oaire.citation.endPage262por
oaire.citation.startPage249por
oaire.citation.titleVanadium: The Versatile metalpor
person.familyNameAureliano
person.givenNameManuel
person.identifier584146
person.identifier.ciencia-idAA14-3490-DC5E
person.identifier.orcid0000-0003-4858-3201
person.identifier.ridI-3283-2012
person.identifier.scopus-author-id6603412860
rcaap.rightsopenAccesspor
rcaap.typebookPartpor
relation.isAuthorOfPublicationbb413661-7edd-4b57-8338-33889cfd05db
relation.isAuthorOfPublication.latestForDiscoverybb413661-7edd-4b57-8338-33889cfd05db

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