Repository logo
 
Publication

Protein expression profiles in Bathymodiolus azoricus exposed to cadmium

2019Journal article Restricted access
Simple item page
dc.contributor.authorCompany, Rui
dc.contributor.authorAntúnez, Oreto
dc.contributor.authorCosson, Richard P.
dc.contributor.authorSerafim, M. A.
dc.contributor.authorShillito, Bruce
dc.contributor.authorCajaraville, Miren
dc.contributor.authorBebianno, Maria João
dc.contributor.authorTorreblanca, Amparo
dc.date.accessioned2020-04-07T10:21:53Z
dc.date.available2020-04-07T10:21:53Z
dc.date.issued2019
dc.description.abstractProteomic changes in the "gill-bacteria complex" of the hydrothermal vent mussel B. azoricus exposed to cadmium in pressurized chambers ((Incubateurs Pressurises pour l'Observation en Culture d'Animaux Marins Profonds - IPOCAMP) were analyzed and compared with the non-exposed control group. 2-D Fluorescence Difference Gel Electrophoresis (2D-DIGE) showed that less than 1.5% of the proteome of mussels and symbiotic bacteria were affected by a short-term (24 h) Cd exposure. Twelve proteins of the more abundant differentially expressed proteins of which six were up-regulated and six were down-regulated were excised, digested and identified by mass spectrometry. The identified proteins included structural proteins (actin/actin like proteins), metabolic proteins (calreticulin/calnexin, peptidyl-prolyl cis-trans isomerase, aminotransferase class-III, electron transfer flavoprotein, proteasome, alpha-subunit and carbonic anhydrase) and stress response proteins (chaperone protein htpG, selenium-binding protein and glutathione transferases). All differently expressed proteins are tightly connected to Cd exposure and are affected by oxidative stress. It was also demonstrated that B. azoricus was well adapted to Cd contamination therefore B. azoricus from hydrothermal vent areas may be considered a good bioindicator.pt_PT
dc.description.sponsorshipEVK3CT1999-00003pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.ecoenv.2019.01.031pt_PT
dc.identifier.issn0147-6513
dc.identifier.urihttp://hdl.handle.net/10400.1/13697
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationA PROTEOMIC APPROACH TO THE INVESTIGATION OF MOLECULAR MECHANISMS INVOLVED IN OXIDATIVE STRESS RESISTANCE IN HYDROTHERMAL AND COASTAL MARINE ORGANISMS
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectBacteriapt_PT
dc.subjectCadmiumpt_PT
dc.subjectElectrophoresispt_PT
dc.subjectGene expression regulationpt_PT
dc.subjectGillspt_PT
dc.subjectHydrothermal ventspt_PT
dc.subjectMytilidaept_PT
dc.subjectOxidative stresspt_PT
dc.subjectSymbiosispt_PT
dc.subjectProteomept_PT
dc.subjectGelpt_PT
dc.titleProtein expression profiles in Bathymodiolus azoricus exposed to cadmiumpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleA PROTEOMIC APPROACH TO THE INVESTIGATION OF MOLECULAR MECHANISMS INVOLVED IN OXIDATIVE STRESS RESISTANCE IN HYDROTHERMAL AND COASTAL MARINE ORGANISMS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F26399%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAR%2F00350%2F2013/PT
oaire.citation.endPage630pt_PT
oaire.citation.startPage621pt_PT
oaire.citation.titleEcotoxicology and Environmental Safetypt_PT
oaire.citation.volume171pt_PT
oaire.fundingStream5876
person.familyNamePereira Serafim
person.familyNameBebianno
person.givenNameMaria Angela
person.givenNameMaria
person.identifier.ciencia-id9C18-090A-5E3D
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0002-1165-0645
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0e42fc03-7052-438d-952b-b3caa5bdc36a
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery0e42fc03-7052-438d-952b-b3caa5bdc36a
relation.isProjectOfPublication3793dcb2-53f7-464c-bdb8-3fcdd7fc2e6e
relation.isProjectOfPublication3c9090f1-c8fc-4990-8cd8-d61d72eb13f7
relation.isProjectOfPublication.latestForDiscovery3c9090f1-c8fc-4990-8cd8-d61d72eb13f7

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Company et al 2019 EES.pdf
Size:
1.21 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.46 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIM2-Artigos (em revistas ou actas indexadas)
FCT2-Artigos (em revistas ou actas indexadas)