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Random mutagenesis as a promising tool for microalgal strain improvement towards industrial production

2022-06-30Journal article Open access
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dc.contributor.authorTrovão Dos Santos, Mafalda
dc.contributor.authorSchüler, Lisa M.
dc.contributor.authorMachado, Adriana
dc.contributor.authorBombo, Gabriel
dc.contributor.authorNavalho, Sofia
dc.contributor.authorBarros, Ana
dc.contributor.authorPereira, Hugo
dc.contributor.authorSilva, Joana
dc.contributor.authorFreitas, Filomena
dc.contributor.authorVarela, João
dc.date.accessioned2022-07-26T10:34:39Z
dc.date.available2022-07-26T10:34:39Z
dc.date.issued2022-06-30
dc.date.updated2022-07-25T16:32:37Z
dc.description.abstractMicroalgae have become a promising novel and sustainable feedstock for meeting the rising demand for food and feed. However, microalgae-based products are currently hindered by high production costs. One major reason for this is that commonly cultivated wildtype strains do not possess the robustness and productivity required for successful industrial production. Several strain improvement technologies have been developed towards creating more stress tolerant and productive strains. While classical methods of forward genetics have been extensively used to determine gene function of randomly generated mutants, reverse genetics has been explored to generate specific mutations and target phenotypes. Site-directed mutagenesis can be accomplished by employing different gene editing tools, which enable the generation of tailor-made genotypes. Nevertheless, strategies promoting the selection of randomly generated mutants avoid the introduction of foreign genetic material. In this paper, we review different microalgal strain improvement approaches and their applications, with a primary focus on random mutagenesis. Current challenges hampering strain improvement, selection, and commercialization will be discussed. The combination of these approaches with high-throughput technologies, such as fluorescence-activated cell sorting, as tools to select the most promising mutants, will also be discussed.pt_PT
dc.description.sponsorshipALG-01-0247-FEDER-069961
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMarine Drugs 20 (7): 440 (2022)pt_PT
dc.identifier.doi10.3390/md20070440pt_PT
dc.identifier.eissn1660-3397
dc.identifier.urihttp://hdl.handle.net/10400.1/18094
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationCentre for Marine and Environmental Research
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectReverse and forward geneticspt_PT
dc.subjectSelection methodspt_PT
dc.subjectFluorescence-activated cell sortingpt_PT
dc.subjectAdaptive laboratory evolutionpt_PT
dc.subjectGenetic engineeringpt_PT
dc.titleRandom mutagenesis as a promising tool for microalgal strain improvement towards industrial productionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.citation.issue7pt_PT
oaire.citation.startPage440pt_PT
oaire.citation.titleMarine Drugspt_PT
oaire.citation.volume20pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameTrovão dos Santos
person.familyNameSchüler
person.familyNameNavalho
person.familyNamePereira
person.familyNameVarela
person.givenNameMafalda
person.givenNameLisa
person.givenNameSofia
person.givenNameHugo
person.givenNameJoão
person.identifier282781
person.identifier.ciencia-idD912-559A-7706
person.identifier.ciencia-idE01F-9B43-4F24
person.identifier.ciencia-id7711-2228-F49F
person.identifier.ciencia-id331E-84FD-2F34
person.identifier.orcid0000-0002-9465-7681
person.identifier.orcid0000-0003-0961-5397
person.identifier.orcid0000-0001-9380-4945
person.identifier.orcid0000-0002-1369-2099
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridE-9360-2015
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id54080259900
person.identifier.scopus-author-id55741416000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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