Publication
Single strand conformation polymorphism (SSCP) detection in six genes in the Portuguese indigenous sheep breed "Churra da Terra Quente"
| dc.contributor.author | Bastos, Estela | |
| dc.contributor.author | Cravador, A. | |
| dc.contributor.author | Azevedo, Jorge | |
| dc.contributor.author | Guedes-Pinto, Henrique | |
| dc.date.accessioned | 2012-06-09T10:14:17Z | |
| dc.date.available | 2012-06-09T10:14:17Z | |
| dc.date.issued | 2001 | |
| dc.date.updated | 2012-06-03T08:27:25Z | |
| dc.description.abstract | Evaluation of the genetic diversity for six genes in forty animals of the Portuguese indigenous sheep breed (Ovis aries) “Churra da Terra Quente” was done. A non-radioactive method to allow single-strand conformation polymorphism (SSCP) detection was optimised, starting from genomic DNAand PCR amplification of seven fragments: exon 1 of the a-lactalbumin gene; exons 10 and 11 of the as1-casein gene; exon 7 of the b-casein gene; exon 4 of the k-casein gene; exons 4 and 5 of the growth hormone gene and exon 6 of the growth hormone receptor gene. Polymorphisms were detected in five of the PCR products. Only k-casein and growth hormone receptor were monomorphic. a-lactalbumin and as1-casein exons showed three conformational patterns, b-casein and growth hormone exon 4 showed two electrophoretic patterns and growth hormone exon 5 showed five conformational patterns. These data provide evidence that “Churra da Terra Quente” has a high genetic variability, which opens interesting prospects for future selection programs and also for preservation strategies. Also, our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability. | por |
| dc.identifier.citation | Bastos, Estela; Cravador, Alfredo; Azevedo, Jorge; Guedes-Pinto, Henrique. Single strand conformation polymorphism (SSCP) detection in six genes in the Portuguese indigenous sheep breed "Churra da Terra Quente". Biotechnology, Agronomy, Society and Environment, 5, 1, 7-15, 2001. | por |
| dc.identifier.issn | 1370-6233 | |
| dc.identifier.other | AUT: ACR00659; | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/1209 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.subject | Single Strand Conformation Polymorphism – SSCP | por |
| dc.subject | Genetic variability | por |
| dc.subject | Growth hormone | por |
| dc.subject | Lactoproteins | por |
| dc.subject | Sheep | por |
| dc.subject | Landraces | por |
| dc.title | Single strand conformation polymorphism (SSCP) detection in six genes in the Portuguese indigenous sheep breed "Churra da Terra Quente" | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 15 | por |
| oaire.citation.issue | 5(1) | por |
| oaire.citation.startPage | 7 | por |
| oaire.citation.title | Biotechnology, Agronomy, Society and Environment | por |
| person.familyName | Cravador | |
| person.givenName | Alfredo | |
| person.identifier.orcid | 0000-0002-9831-9815 | |
| person.identifier.rid | K-7247-2012 | |
| person.identifier.scopus-author-id | 6602537257 | |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | 8c84a9e6-6b7f-4b50-93a2-152c85901722 | |
| relation.isAuthorOfPublication.latestForDiscovery | 8c84a9e6-6b7f-4b50-93a2-152c85901722 |