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Functional expression of the human CHIP28 water channel in a yeast secretory mutant

dc.contributor.authorLaizé, Vincent
dc.contributor.authorRousselet, G.
dc.contributor.authorVerbavatz, J. M.
dc.contributor.authorBerthonaud, V.
dc.contributor.authorGobin, R.
dc.contributor.authorRoudier, N.
dc.contributor.authorAbrami, L.
dc.contributor.authorRipoche, P.
dc.contributor.authorTacnet, F.
dc.date.accessioned2014-06-06T13:21:37Z
dc.date.available2014-06-06T13:21:37Z
dc.date.issued1995-10-16
dc.date.updated2014-06-03T10:28:46Z
dc.description.abstractThe temperature-sensitive Saccharomyces cerevisiae mutant strain NY17, deficient in the secretory pathway (sec6-4 mutation), is used for the heterologous expression of the human CHIP28 water channel. After a heat-shock, the protein is present in partially purified post-golgi secretory vesicles, lmmunodetection and water transport studies, directly made on the vesicles, showed that CHIP28 is highly expressed and active in the yeast membranes.por
dc.identifier.citationLaizé V; Rousselet G; Verbavatz JM; Berthonaud V; Gobin R; Roudier N; Abrami L; Ripoche P; Tacnet F. Functional expression of the human CHIP28 water channel in a yeast secretory mutant, FEBS Letters, 373, 3, 269-274, 1995.por
dc.identifier.doihttp://dx.doi.org/10.1016/0014-5793(95)01060-R
dc.identifier.issn0014-5793
dc.identifier.urihttp://hdl.handle.net/10400.1/4216
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/001457939501060Rpor
dc.subjectSaccharomyces cerevisiae sec6-4 nutantpor
dc.subjectPost-golgi secretory vesiclespor
dc.subjectWater transportpor
dc.subjectAQP1por
dc.titleFunctional expression of the human CHIP28 water channel in a yeast secretory mutantpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage274por
oaire.citation.issue3por
oaire.citation.startPage269por
oaire.citation.titleFEBS Letterspor
oaire.citation.volume373por
person.familyNameLaizé
person.givenNameVincent
person.identifier.orcid0000-0001-9565-9198
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id6602982778
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublication.latestForDiscoveryb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f

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