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Respirable konjac glucomannan microparticles as antitubercular drug carriers: Effects of in vitro and in vivo interactions

2023-09Journal article Restricted access
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dc.contributor.authorGuerreiro, Filipa
dc.contributor.authorPontes, Jorge Filipe
dc.contributor.authorGaspar, Maria Manuela
dc.contributor.authorRosa Da Costa, Ana
dc.contributor.authorFaleiro, Maria Leonor
dc.contributor.authorGrenha, Ana
dc.date.accessioned2023-09-22T17:10:42Z
dc.date.available2023-09-22T17:10:42Z
dc.date.issued2023-09
dc.description.abstractPulmonary delivery of drugs is potentially beneficial in the context of lung disease, maximising drug concentrations in the site of action. A recent work proposed spray-dried konjac glucomannan (KGM) microparticles as antitubercular drug (isoniazid and rifabutin) carriers to treat pulmonary tuberculosis. The present work explores in vitro and in vivo effects of these microparticles, focusing on the ability for macrophage uptake, the exhibited antibacterial activity and safety issues. Efficient uptake of KGM microparticles by macrophages was demonstrated in vitro, while the antitubercular activity of the model drugs against Mycobacterium bovis was not affected by microencapsulation in KGM microparticles. Despite the good indications provided by the developed system, KGM is not yet approved for pulmonary applications, which is a limiting characteristic. To reinforce the available data on the performance of the material, safety parameters were evaluated both in vitro and in vivo, showing promising results. No significant cell toxicity was observed at concentrations considered realistic for lung delivery approaches (up to 125 & mu;g/mL) when lung epithelial cells and macrophages were exposed to KGM microparticles (both drug-loaded and unloaded). Finally, no signs of systemic or lung inflammatory response were detected in mice after receiving 10 administrations of unloaded KGM microparticles.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.ijbiomac.2023.125838pt_PT
dc.identifier.eissn1879-0003
dc.identifier.urihttp://hdl.handle.net/10400.1/20013
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationFighting TB: Development of microparticulate systems to target alveolar macrophages in tuberculosis therapy
dc.relationAlgarve Centre for Marine Sciences
dc.relationKonjac glucomannan microcarriers: an application in the treatment of pulmonary tuberculosis
dc.subjectKonjac glucomannan microparticlespt_PT
dc.subjectMacrophagespt_PT
dc.subjectSafety profilept_PT
dc.titleRespirable konjac glucomannan microparticles as antitubercular drug carriers: Effects of in vitro and in vivo interactionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleFighting TB: Development of microparticulate systems to target alveolar macrophages in tuberculosis therapy
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleKonjac glucomannan microcarriers: an application in the treatment of pulmonary tuberculosis
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FDTP-FTO%2F0094%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F115628%2F2016/PT
oaire.citation.startPage125838pt_PT
oaire.citation.titleInternational Journal of Biological Macromoleculespt_PT
oaire.citation.volume248pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameGuerreiro
person.familyNamePontes
person.familyNameRosa da Costa
person.familyNameFaleiro
person.familyNameGrenha
person.givenNameFilipa
person.givenNameJorge Filipe
person.givenNameAna M
person.givenNameMaria Leonor
person.givenNameAna
person.identifier305029
person.identifier252666
person.identifier.ciencia-idBF1D-7EFA-19BC
person.identifier.ciencia-idCB16-5CBD-8B89
person.identifier.ciencia-idA418-A85E-5DB1
person.identifier.ciencia-id281D-5E01-F09A
person.identifier.ciencia-id091C-0D58-7225
person.identifier.orcid0000-0001-6224-1553
person.identifier.orcid0000-0002-6447-0242
person.identifier.orcid0000-0003-0225-9537
person.identifier.orcid0000-0002-3878-6948
person.identifier.orcid0000-0002-2136-1396
person.identifier.ridE-2165-2012
person.identifier.ridH-1392-2017
person.identifier.scopus-author-id57195033461
person.identifier.scopus-author-id53986075100
person.identifier.scopus-author-id6507927018
person.identifier.scopus-author-id8607930100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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