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Valorisation of gilthead seabream by-products through recovery of antimicrobial proteins for active biopolymer formulations

2026-01Artigo científico Acesso restrito
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datacite.subject.sdg14:Proteger a Vida Marinha
datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
dc.contributor.authorMaurizzi, Enrico
dc.contributor.authorAnjos Guerreiro, Liliana Isabel Tomé
dc.contributor.authorBigi, Francesco
dc.contributor.authorQuartieri, Andrea
dc.contributor.authorMateus, Ana Patrícia
dc.contributor.authorVolpelli, Luisa Antonella
dc.contributor.authorPulvirenti, Andrea
dc.contributor.authorPower, Deborah Mary
dc.date.accessioned2026-03-17T12:55:48Z
dc.date.available2026-03-17T12:55:48Z
dc.date.issued2026-01
dc.description.abstractIn the seafish sector, industrial processing and by-catch currently lead to the waste of over 36 % of global fish production by weight. This is largely due to insufficient revalorization of by-products and the underdevelopment of sustainable practices to manage these discarded volumes, which are often disposed of or released into the environment, contributing to pollution. In this study, antimicrobial proteins were extracted from fish by-products for incorporation into biopolymer formulations. Specifically, the focus was on lysozyme, which was targeted using a molecular-proteomic approach. Protein extractions were conducted at various pH levels from Gilthead seabream (Sparus aurata) tissues (skin with scales and mucus, liver, and intestine) normally discarded during processing, to assess recovery of proteins from the selected tissues. The extracted proteins were separated using mild ion-exchange chromatography, followed by quantification and qualitative analysis via SDS-PAGE. The expression levels of lysozyme types-g and-c were quantified through Real-Time qPCR. The antimicrobial activity of the extracted proteins was assessed against Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli) bacteria using a Minimal Inhibitory Concentration (MIC) assay. The proteins were subsequently incorporated into biodegradable film-forming solutions based on hydroxypropyl methylcellulose/chitosan and hydroxypropyl methylcellulose/guar gum mixtures. These films were further tested against the same human pathogens. The results demonstrate the feasibility of extracting proteins from fish by-products using a non-targeted buffer pH extraction approach, which, even without further chromatographic purification, exhibited promising intrinsic antimicrobial activity for potential applications in the food industry.eng
dc.description.sponsorshipUID/04326/2025, UID/PRR/04326/2025, EMBRC.PT. ALG-01-0145-FEDER-022121
dc.identifier.doi10.1016/j.ijbiomac.2025.150014
dc.identifier.issn0141-8130
dc.identifier.urihttp://hdl.handle.net/10400.1/28444
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationOptImization of novel value CHains for fish and seafood by developing an integraTed sustainable approacH for improved qualitY, safety and waSte reduction
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.rights.uriN/A
dc.subjectBy-products
dc.subjectBiopolymers
dc.subjectFish waste
dc.subjectAntimicrobial
dc.subjectLysozyme
dc.titleValorisation of gilthead seabream by-products through recovery of antimicrobial proteins for active biopolymer formulationseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumber872217
oaire.awardNumberLA/P/0101/2020
oaire.awardTitleOptImization of novel value CHains for fish and seafood by developing an integraTed sustainable approacH for improved qualitY, safety and waSte reduction
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/872217/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.startPage150014
oaire.citation.titleInternational Journal of Biological Macromolecules
oaire.citation.volume339
oaire.fundingStreamH2020
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameAnjos Guerreiro
person.familyNameMateus
person.familyNamePower
person.givenNameLiliana Isabel Tomé
person.givenNameAna Patrícia
person.givenNameDeborah Mary
person.identifierAAK-3800-2020
person.identifier.ciencia-id6D18-2D1C-A45C
person.identifier.ciencia-id2816-E389-626E
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-9474-522X
person.identifier.orcid0000-0003-2045-1047
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id8575757700
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication721b5dca-ad42-40f8-9731-517eb5db009e
relation.isAuthorOfPublication74cac9a6-f4f8-438b-a60e-a2b4d3312102
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery721b5dca-ad42-40f8-9731-517eb5db009e
relation.isProjectOfPublication89c67688-3498-4a31-bcfd-c8142621e215
relation.isProjectOfPublication794d4c77-c731-471e-bc96-5a41dcd3d872
relation.isProjectOfPublication.latestForDiscovery89c67688-3498-4a31-bcfd-c8142621e215

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