Valorisation of gilthead seabream by-products through recovery of antimicrobial proteins for active biopolymer formulations

Maurizzi, Enrico; Anjos Guerreiro, Liliana Isabel Tomé; Bigi, Francesco; Quartieri, Andrea; Mateus, Ana Patrícia; Volpelli, Luisa Antonella; Pulvirenti, Andrea; Power, Deborah Mary

http://hdl.handle.net/10400.1/28444

Utilize este identificador para referenciar este registo.

Nome:	Descrição:	Tamanho:	Formato:
1-s2.0-S0141813025105710-main.pdf		8.16 MB	Adobe PDF	Ver/Abrir

Contacte-nos

Autores

Maurizzi, Enrico

Anjos Guerreiro, Liliana Isabel Tomé

Bigi, Francesco

Quartieri, Andrea

Mateus, Ana Patrícia

Volpelli, Luisa Antonella

Pulvirenti, Andrea

Power, Deborah Mary

Resumo(s)

In the seafish sector, industrial processing and by-catch currently lead to the waste of over 36 % of global fish production by weight. This is largely due to insufficient revalorization of by-products and the underdevelopment of sustainable practices to manage these discarded volumes, which are often disposed of or released into the environment, contributing to pollution. In this study, antimicrobial proteins were extracted from fish by-products for incorporation into biopolymer formulations. Specifically, the focus was on lysozyme, which was targeted using a molecular-proteomic approach. Protein extractions were conducted at various pH levels from Gilthead seabream (Sparus aurata) tissues (skin with scales and mucus, liver, and intestine) normally discarded during processing, to assess recovery of proteins from the selected tissues. The extracted proteins were separated using mild ion-exchange chromatography, followed by quantification and qualitative analysis via SDS-PAGE. The expression levels of lysozyme types-g and-c were quantified through Real-Time qPCR. The antimicrobial activity of the extracted proteins was assessed against Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli) bacteria using a Minimal Inhibitory Concentration (MIC) assay. The proteins were subsequently incorporated into biodegradable film-forming solutions based on hydroxypropyl methylcellulose/chitosan and hydroxypropyl methylcellulose/guar gum mixtures. These films were further tested against the same human pathogens. The results demonstrate the feasibility of extracting proteins from fish by-products using a non-targeted buffer pH extraction approach, which, even without further chromatographic purification, exhibited promising intrinsic antimicrobial activity for potential applications in the food industry.