Chemical characterization of volatile and non-volatile constituents of aqueous extracts from euphorbia officinarum and E. resinifera: influence of extraction conditions on antioxidant and α-glucosidase inhibitory activities

BOUTOUB, OUMAIMA; El Ghadraoui, Lahsen; El-Guendouz, Soukaina; Aazza, Smail; Figueiredo, Ana Cristina; Silva, José Paulo da; Miguel, Maria da Graça

http://hdl.handle.net/10400.1/29478

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Autores

BOUTOUB, OUMAIMA

El Ghadraoui, Lahsen

El-Guendouz, Soukaina

Aazza, Smail

Figueiredo, Ana Cristina

Silva, José Paulo da

Miguel, Maria da Graça

Resumo(s)

This study performed a chemical characterization of Euphorbia officinarum and Euphorbia resinifera volatile and non-volatile aqueous extracts. It was also evaluated how extraction variables (extraction time and plant-tosolvent ratio, PSR) influenced the chemical composition and biological responses of the aqueous extracts of four populations of E. officinarum and one population of E. resinifera. The experimental results were fitted using second-order polynomial equations for all response variables. The volatiles (essential oils) were isolated by hydrodistillation and analyzed for quantification and component identification, by gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS), respectively. The volatile fraction of E. officinarum contained higher percentages of oxygenated monoterpenes and phenylpropanoids, whereas palmitic acid predominated in E. resinifera. Liquid chromatography-mass spectrometry (LC-MS) was used to analyze the aqueous extract of both species and the tentatively annotated compounds included particularly flavonoids, coumarins, oxylipins, and aliphatic dicarboxylic acids. Two-way ANOVA and response surface methodology highlighted that PSR was generally the main factor affecting the measured responses, whereas the influence of extraction time was response- and population-dependent. In addition, Pearson correlation and principal component analyses showed that the in vitro biological activities were more dependent on phytochemical composition than on total phenolic content.

Palavras-chave

Euphorbia officinarum Euphorbia resinifera Aqueous extracts Response surface methodology Phytochemical profiling Antioxidant activity α-glucosidase inhibition