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Selection criteria of Zebrafish male donors for sperm cryopreservation

2019Journal article Open access
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dc.contributor.authorDiogo, Patricia
dc.contributor.authorMartins, Gil
dc.contributor.authorEufrásio, Ana
dc.contributor.authorSilva, Tomé
dc.contributor.authorCabrita, Elsa
dc.contributor.authorGavaia, Paulo
dc.date.accessioned2020-07-29T12:13:05Z
dc.date.available2020-07-29T12:13:05Z
dc.date.issued2019
dc.description.abstractSelection criteria for sperm cryopreservation are highly relevant in zebrafish since sperm quality is particularly variable in this species. Successful cryopreservation depends on high-quality sperm, which can only be ensured by the selection of breeders. Consequently, male selection and management are a priority to improve cryopreservation, and therefore, this study aimed to characterize optimal age and sperm collection frequency in zebrafish. For this purpose, males from wild type (AB) and from a transgenic line [Tg(runx2:eGFP)] were sampled at 6, 8, 12, and 14 months. For each age, sperm were collected at time 0 followed by samplings at 2, 7, and 14 days of rest. Sperm quality was assessed according to motility and membrane viability parameters. Quality assessment showed that Tg(runx2:eGFP) displayed significantly higher motility than AB and younger males showed higher motility in both lines. Sperm collection frequency affected membrane viability. While AB fish recovered sperm viability after 14 days of rest, Tg(runx2:eGFP) could not recover. Consequently, it may be important to study the sperm quality of each zebrafish line before sperm cryopreservation. Taking into consideration the results achieved in both lines, sperm collection should be performed between 6 and 8 months of age with a minimum collection interval of 14 days.pt_PT
dc.description.sponsorshipN730984, EBB-EAPA_501/2016, PEst-C/MAR/LA0015/2011pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1089/zeb.2018.1660pt_PT
dc.identifier.issn1545-8547
dc.identifier.urihttp://hdl.handle.net/10400.1/14541
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAge factorspt_PT
dc.subjectAnimalspt_PT
dc.subjectCell membranept_PT
dc.subjectCryopreservationpt_PT
dc.subjectMalept_PT
dc.subjectSperm motilitypt_PT
dc.subjectSpermatozoapt_PT
dc.subjectZebrafishpt_PT
dc.subjectSpecimen handlingpt_PT
dc.titleSelection criteria of Zebrafish male donors for sperm cryopreservationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F97466%2F2013/PT
oaire.citation.endPage196pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage189pt_PT
oaire.citation.titleZebrafishpt_PT
oaire.citation.volume16pt_PT
oaire.fundingStreamSFRH
person.familyNameDiogo
person.familyNameMartins
person.familyNameEufrásio
person.familyNameCabrita
person.familyNameGavaia
person.givenNamePatricia
person.givenNameGil
person.givenNameAna
person.givenNameElsa
person.givenNamePaulo
person.identifier.ciencia-idFD18-ED81-FFB5
person.identifier.ciencia-idD11F-8D8A-8EA1
person.identifier.ciencia-id6514-65E1-6C69
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0002-0804-5014
person.identifier.orcid0000-0002-1344-2954
person.identifier.orcid0000-0002-2753-7874
person.identifier.orcid0000-0003-3864-8841
person.identifier.orcid0000-0002-9582-1957
person.identifier.ridE-8523-2012
person.identifier.ridA-6470-2011
person.identifier.scopus-author-id36695981300
person.identifier.scopus-author-id26645697000
person.identifier.scopus-author-id6507104377
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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