Repository logo
 
Publication

New bacterial strains for ibuprofen biodegradation: drug removal, transformation, and potential catabolic genes

2024-08Journal article Open access
Simple item page
dc.contributor.authorLara-Moreno, Alba
dc.contributor.authorCosta, Maria Clara
dc.contributor.authorVargas‐Villagomez, Ayleen
dc.contributor.authorCarlier, Jorge
dc.date.accessioned2024-11-02T11:06:43Z
dc.date.available2024-11-02T11:06:43Z
dc.date.issued2024-08
dc.description.abstractIbuprofen (IBU) is a significant contaminant frequently found in wastewater treatment plants due to its widespread use and limited removal during treatment processes. This leads to its discharge into the environment, causing considerable environmental concerns. The use of microorganisms has recently been recognized as a sustainable method for mitigating IBU contamination in wastewater. In this study, new bacteria capable of growing in a solid medium with IBU as the only carbon source and removing IBU from a liquid medium were isolated from environmental samples, including soil, marine, mine, and olive mill wastewater. Four bacterial strains, namely Klebsiella pneumoniae TIBU2.1, Klebsiella variicola LOIBU1.1, Pseudomonas aeruginosa LOIBU1.2, and Mycolicibacterium aubagnense HPB1.1, were identified through 16S rRNA gene sequencing. These strains demonstrated significant IBU removal efficiencies, ranging from 60 to 100% within 14 days, starting from an initial IBU concentration of 5 mg per litre. These bacteria have not been previously reported in the literature as IBU degraders, making this work a valuable contribution to further studies in the field of bioremediation in environments contaminated by IBU. Based on the IBU removal results, the most promising bacteria, K. pneumoniae TIBU2.1 and M. aubagnense HPB1.1, were selected for an in silico analysis to identify genes potentially involved in IBU biodegradation. Interestingly, in the tests with TIBU2.1, a peak of IBU transformation product(s) was detected by high-performance liquid chromatography, while in the tests with HPB1.1, it was not detected. The emerging peak was analysed by liquid chromatography-mass spectrometry, indicating the presence of possible conjugates between intermediates of IBU biodegradation. The proteins encoded on their whole-genome sequences were aligned with proteins involved in an IBU-degrading pathway reported in bacteria with respective catabolic genes. The analysis indicated that strain HPB1.1 possesses genes encoding proteins similar to most enzymes reported associated with the IBU metabolic pathways used as reference bacteria, while strain TIBU2.1 has genes encoding proteins similar to enzymes involved in both the upper and the lower part of that pathway. Notably, in the tests with the strain having more candidate genes encoding IBU-catabolic enzymes, no IBU transformation products were detected, while in the tests with the strain having fewer of these genes, detection occurred. This study explores the use of bacteria to mitigate ibuprofen (IBU) contamination in wastewater. Four new bacterial strains were identified that can grow with IBU as the only carbon source and demonstrated significant IBU removal efficiencies. In-depth analysis revealed that two strains, Klebsiella pneumoniae TIBU2.1 and Mycolicibacterium aubagnense HPB1.1, possess genes encoding proteins similar to enzymes involved in IBU degradation, providing a promising avenue for bioremediation in IBU-contaminated environments. imageeng
dc.description.sponsorshipALG-01-0145-FEDER-022231; ALG-01-0145-FEDER-022121
dc.identifier.doi10.1111/1758-2229.13320
dc.identifier.issn1758-2229
dc.identifier.urihttp://hdl.handle.net/10400.1/26195
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relationCentre for Marine and Environmental Research
dc.relationBioaugmentation and conjugative plasmid transference using bacteria from extreme environments to enhance biodegradation of recalcitrant pollutants in WWTP granular sludge
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relation.ispartofEnvironmental Microbiology Reports
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleNew bacterial strains for ibuprofen biodegradation: drug removal, transformation, and potential catabolic geneseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardTitleBioaugmentation and conjugative plasmid transference using bacteria from extreme environments to enhance biodegradation of recalcitrant pollutants in WWTP granular sludge
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCTA-AMB%2F7782%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.citation.issue4
oaire.citation.startPagee13320
oaire.citation.titleEnvironmental Microbiology Reports
oaire.citation.volume16
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameLara-Moreno
person.familyNameCosta
person.familyNameCarlier
person.givenNameAlba
person.givenNameMaria Clara
person.givenNameJorge
person.identifier.ciencia-id7110-A28E-3C8A
person.identifier.ciencia-id691D-A1BB-15CC
person.identifier.orcid0000-0002-5741-7662
person.identifier.orcid0000-0003-1340-5237
person.identifier.orcid0000-0003-0675-2716
person.identifier.ridM-6189-2013
person.identifier.scopus-author-id35354952400
person.identifier.scopus-author-id7102166949
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication300c1ba7-9297-4afb-b2a1-3201af0cd639
relation.isAuthorOfPublication11c29f10-6e2b-40d8-9c74-2cc45be6d7a7
relation.isAuthorOfPublicationd4af2b00-61d6-4fd9-b05d-9ed235b5c7de
relation.isAuthorOfPublication.latestForDiscovery300c1ba7-9297-4afb-b2a1-3201af0cd639
relation.isProjectOfPublication794d4c77-c731-471e-bc96-5a41dcd3d872
relation.isProjectOfPublication0b09c039-bd11-41d7-bebf-ae11435c521d
relation.isProjectOfPublicationfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f
relation.isProjectOfPublication15f91d45-e070-47d8-b6b8-efd4de31d9a8
relation.isProjectOfPublication.latestForDiscovery794d4c77-c731-471e-bc96-5a41dcd3d872

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Environ Microbiol Rep - 2024 - Lara‐Moreno - New bacterial strains for ibuprofen biodegradation Drug removal .pdf
Size:
4.9 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.46 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)
FCT2-Artigos (em revistas ou actas indexadas)