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In situ monitoring of chlorophyll a fluorescence in Nannochloropsis oceanica cultures to assess photochemical changes and the onset of lipid accumulation during nitrogen deprivation

2021Journal article Restricted access
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dc.contributor.authorCarneiro, Mariana
dc.contributor.authorChini Zittelli, Graziella
dc.contributor.authorCicchi, Bernardo
dc.contributor.authorTouloupakis, Eleftherios
dc.contributor.authorFaraloni, Cecilia
dc.contributor.authorMaia, Inês Beatriz
dc.contributor.authorPereira, Hugo
dc.contributor.authorSantos, Tamára
dc.contributor.authorMalcata, Francisco X.
dc.contributor.authorOtero, Ana
dc.contributor.authorVarela, João
dc.contributor.authorTorzillo, Giuseppe
dc.date.accessioned2021-09-20T09:10:04Z
dc.date.available2021-09-20T09:10:04Z
dc.date.issued2021
dc.description.abstractIn situ chlorophyll a fluorescence measurements were applied to monitor changes in the photochemical variables of Nannochloropsis oceanica cultures under nitrogen-deplete and nitrogen-replete (control) conditions. In addition, growth, lipid, fatty acid, and pigment contents were also followed. In the control culture, growth was promoted along with pigment content, electron transport rate (ETR), and polyunsaturated fatty acids, while total lipid content and fatty acid saturation level diminished. Under nitrogen-deplete conditions, the culture showed a higher de-epoxidation state of the xanthophyll cycle pigments. Fast transients revealed a poor processing efficiency for electron transfer beyond Q(A), which was in line with the low ETR due to nitrogen depletion. Lipid content and the de-epoxidation state were the first biochemical variables triggered by the change in nutrient status, which coincided with a 20% drop in the in situ effective quantum yield of PSII (Delta F'/F-m'), and a raise in the V-j measurements. A good correlation was found between the changes in Delta F'/F-m' and lipid content (r = -0.96, p < 0.01). The results confirm the reliability and applicability of in situ fluorescence measurements to monitor lipid induction in N. oceanica.pt_PT
dc.description.sponsorshipES1408; PTDC/BBB-EBB/1374/2014-POCI-01-0145-FEDER-016640pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/bit.27906pt_PT
dc.identifier.eissn1097-0290
dc.identifier.urihttp://hdl.handle.net/10400.1/17126
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationSustainable Algae Biorefinery for Agriculture aNd Aquaculture
dc.subjectIn situ chlorophyll a fluorescencept_PT
dc.subjectNannochloropsispt_PT
dc.subjectNutrient stresspt_PT
dc.subjectPhotosynthesispt_PT
dc.titleIn situ monitoring of chlorophyll a fluorescence in Nannochloropsis oceanica cultures to assess photochemical changes and the onset of lipid accumulation during nitrogen deprivationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSustainable Algae Biorefinery for Agriculture aNd Aquaculture
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/67418/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157544/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/727874/EU
oaire.citation.titleBiotechnology and Bioengineeringpt_PT
oaire.fundingStreamPOR_NORTE
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamH2020
person.familyNameMaia
person.familyNamePereira
person.givenNameInês Beatriz
person.givenNameHugo
person.identifier282781
person.identifier.ciencia-idE017-3A05-FF0A
person.identifier.ciencia-id331E-84FD-2F34
person.identifier.orcid0000-0002-7509-2216
person.identifier.orcid0000-0002-1369-2099
person.identifier.ridE-9360-2015
person.identifier.scopus-author-id54080259900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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