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Osteocalcin and Matrix Gla Protein in developing teleost teeth. Identification of sites of mRNA and protein accumulation at single cell resolution
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Advisor(s)
Abstract(s)
In this study, the tissue distribution and accumulation
of osteocalcin or bone Gla protein (BGP) and
matrix Gla protein (MGP) were determined during tooth
development in a teleost fish, Argyrosomus regius. In this
species, the presence ofBGP andMGPmRNAin teethwas
revealed by in situ hybridization. mRNA for BGP was
detected in the odontoblasts as well as in its cytoplasmic
processes emerging through dentinal tubules,whilemRNA
for MGP was expressed in the enamel portion within the
apical portion of the elongated cell bodies of enameloblasts,
adjacent to the root of the teeth as well as in cells
within the pulpal space. Immunolocalization of BGP and
MGP demonstrated that these proteins accumulate mainly
in the mineralized dentin or in enameloblastic processes,
confirming in situ hybridization results. In this study, we
examined for the first time the localization of both BGP
and MGP gene expression and protein accumulation
within the different regions of the vertebrate tooth. We
clearly demonstrated that although the overall pattern of
BGP andMGPgene expression and protein accumulation
in A. regius teeth was in general agreement to what is
known for other vertebrates such as rats or rodents, our
study provided novel information and highlighted some
species-differences between fish and higher vertebrates.
Description
Keywords
Osteocalcin BGP Matrix Gla Protein MGP Teleot fish Immunohistochemistry Teeth In situ hybridization
Citation
Ortiz-Delgado, J. B., Simes, D.C., Gavaia, P.J., Sarasquete, C. and Cancela, M.L. 2005. Osteocalcin and Matrix Gla Protein in developing teleost teeth. Identification of sites of mRNA and protein accumulation at single cell resolution. Histochemistry and Cell Biology 124(2):123-130.
