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Osteocalcin and Matrix Gla Protein in developing teleost teeth. Identification of sites of mRNA and protein accumulation at single cell resolution

2005Journal article Unknown
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dc.contributor.authorOrtiz-Delgado, J. B.
dc.contributor.authorSimes, D
dc.contributor.authorGavaia, Paulo J.
dc.contributor.authorSarasquete, C.
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-06-25T13:39:17Z
dc.date.available2014-06-25T13:39:17Z
dc.date.issued2005
dc.date.updated2014-05-30T10:26:46Z
dc.description.abstractIn this study, the tissue distribution and accumulation of osteocalcin or bone Gla protein (BGP) and matrix Gla protein (MGP) were determined during tooth development in a teleost fish, Argyrosomus regius. In this species, the presence ofBGP andMGPmRNAin teethwas revealed by in situ hybridization. mRNA for BGP was detected in the odontoblasts as well as in its cytoplasmic processes emerging through dentinal tubules,whilemRNA for MGP was expressed in the enamel portion within the apical portion of the elongated cell bodies of enameloblasts, adjacent to the root of the teeth as well as in cells within the pulpal space. Immunolocalization of BGP and MGP demonstrated that these proteins accumulate mainly in the mineralized dentin or in enameloblastic processes, confirming in situ hybridization results. In this study, we examined for the first time the localization of both BGP and MGP gene expression and protein accumulation within the different regions of the vertebrate tooth. We clearly demonstrated that although the overall pattern of BGP andMGPgene expression and protein accumulation in A. regius teeth was in general agreement to what is known for other vertebrates such as rats or rodents, our study provided novel information and highlighted some species-differences between fish and higher vertebrates.por
dc.description.sponsorshipPRAXIS XXI BD/99/19665; AGL2003- 03558
dc.identifier.citationOrtiz-Delgado, J. B., Simes, D.C., Gavaia, P.J., Sarasquete, C. and Cancela, M.L. 2005. Osteocalcin and Matrix Gla Protein in developing teleost teeth. Identification of sites of mRNA and protein accumulation at single cell resolution. Histochemistry and Cell Biology 124(2):123-130.por
dc.identifier.doihttp://dx.doi.org/10.1007/s00418-005-0015-y
dc.identifier.otherAUT: LCA00739; AUT: DSI00813;
dc.identifier.urihttp://hdl.handle.net/10400.1/4508
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relationCCMARpor
dc.relationINSIGHTS INTO THE MECHANISM OF VASCULAR CALCIFICATION: STUDIES TOWARDS THE ESTABLISHMENT OF AN IN VITRO MODEL OF VASCULAR CALCIFICATION IN LOWER VERTEBRATES
dc.subjectOsteocalcinpor
dc.subjectBGPpor
dc.subjectMatrix Gla Proteinpor
dc.subjectMGPpor
dc.subjectTeleot fishpor
dc.subjectImmunohistochemistrypor
dc.subjectTeethpor
dc.subjectIn situ hybridizationpor
dc.titleOsteocalcin and Matrix Gla Protein in developing teleost teeth. Identification of sites of mRNA and protein accumulation at single cell resolutionpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleINSIGHTS INTO THE MECHANISM OF VASCULAR CALCIFICATION: STUDIES TOWARDS THE ESTABLISHMENT OF AN IN VITRO MODEL OF VASCULAR CALCIFICATION IN LOWER VERTEBRATES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCTI/SFRH%2FBPD%2F7151%2F2001/PT
oaire.citation.endPage130por
oaire.citation.startPage123por
oaire.citation.titleHistochemistry and Cell Biologypor
oaire.citation.volume124por
oaire.fundingStreamPOCTI
person.familyNameSimes
person.familyNameGavaia
person.familyNameCancela
person.givenNameDina
person.givenNamePaulo
person.givenNameM. Leonor
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0002-5145-4753
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridN-2789-2014
person.identifier.ridA-6470-2011
person.identifier.scopus-author-id7201884723
person.identifier.scopus-author-id6507104377
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication9dca2139-21a4-4d59-aaf7-531f1033a58e
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication.latestForDiscovery31bac96d-1d01-4b8f-8f2b-9da4be31ea41
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